响应面法优化巴戟天低聚糖提取工艺

Optimization of the Extraction Technology for Oligosaccharides of Morinda officinalis by Response Surface Methodology

目的:优化巴戟天低聚糖提取工艺。方法:以液料比、提取时间和提取温度为考察因素,以巴戟天低聚糖提取率为评价指标,在单因素试验的基础上,采用3因素3水平Box-behnken中心组合试验,建立低聚糖提取得率的二次多项式回归方程,经响应面回归分析得到优化组合条件并进行验证。结果:最优提取工艺条件为液料比23∶1(ml/g)、提取时间1.7 h、提取温度93℃、提取2次。该条件下3次验证试验低聚糖提取率实测均值为10.29%(RSD=0.20%,n=3),与预测值10.37%相比偏差为0.06%。结论:采用响应面法可实现巴戟天低聚糖提取工艺的优化。

OBJECTIVE：To optimize the extraction technology of oligosaccharides of Morinda officinalis. METHODS：The liquid/solid ratio,extraction time and temperature were chosen as factors,and the yield of oligosaccharides was estimated as index.On the basis of single-factor experiments,using a 3-factor,3-level Box-behnken central composite experimental design,two-polynomial regression equation of extraction rate of oligosaccharide was established,and it was analyzed by response surface methodology to obtain the optimum extraction conditions,and the verification test was conducted. RESULTS：The optimum extraction conditions were as follows as material/liquid ratio of 23 ∶ 1（ml/g）,extraction time of 1.7 h,and extraction temperature of 93 ℃,extracting for twice. Under these conditions,the estimated and observed average values of extraction rate of oligosaccharides were10.37% and 10.29%（RSD=0.20%,n=3）,respectively. The deviation value was 0.06%. CONCLUSIONS：The response surface methodology can be used to optimize the extraction process of oligosaccharides of M. officinalis.