断奶犊牛瘤胃细菌16S rDNA文库的构建及多样性分析

Construction and diversity analysis of 16S rDNA library of rumen bacteria in weaned calves

为了研究断奶犊牛瘤胃细菌的多样性,试验采用免培技术方法构建了细菌16S r DNA文库并进行了多样性分析。结果表明:试验构建的断奶犊牛瘤胃16S r DNA基因文库中大部分序列位于低G+C含量细菌门、后壁菌门和拟杆菌门,其中有87.8%与已培养菌的相似性≥97%,但有12.2%的序列为瘤胃未分离培养、鉴定菌。除Pseudobutyrivibrio ruminis、Ruminococcus bromii、Selenomonas sputigena等常见菌外,另有一些细菌较为少见,如Hydrogenoanaerobacterium saccharovorans、Mahella australiensis、Alistipes shahii等。

To study ruminal bacteria diversity of weaned calves, culture - independent methods were used to study the diversity of lumen bacteria in weaned calves. The results showed that most of the sequences from the constructed 16S rDNA library of tureen bacteria in weaned calves ru- men were located at Bacteriophyta with low G ＋ C, Firmicutes, and Bacteroidetes, of which 87.8% sequences of bacterial communities had no less than 97% similarity with those of the cultural bacteria. A total of 12.2% sequences of the bacteria were those of the tureen bacteria without isolation, culture and identification. Except the common bacteria such as Pseudobutyrivibrio ruminis, Ruminococcus bromii, Selenomonas sputi- gena etc. , the other bacteria were relatively rare, such as Hydrogenoanaerobacterium saccharovorans , Mahella australiensis , Alistipes shahii etc.