miR-222在不同病理级别胶质瘤中的表达及对预后判断的价值

Expression and significance of miR-222 in different pathological grade gliomas

目的探讨miR-222在不同病理级别胶质瘤中的表达及其与预后的相关性。方法收集不同病理级别胶质瘤及瘤旁正常组织标本,以定量PCR方法检测miR-222的表达。建立受试者工作特征(ROC)曲线,计算ROC曲线下的面积(AUC)来评估miR-222在胶质瘤组织和瘤旁正常组织中表达差异。通过Kaplan-Meier法为高表达及低表达群体建立生存曲线,并用Log-rank检验评估两组生存曲线的差异。结果 miR-222在胶质瘤组织中的表达显著高于瘤周正常组织,miR-222的最佳临界值为3.23,敏感性为0.78,特异性为0.76,且与胶质瘤的病理级别呈正相关。在生存分析中,高表达miR-222的胶质瘤患者预后较差。结论 miR-222是胶质瘤恶性程度的重要标志物,有望成为胶质瘤预后判断的参考指标。

Objective To analyze the prognostic roles of abnormal expression of miR-222 in different pathological grade gliomas. Methods The expressions of miR-222 were detected by q RT-PCR in glioma tissue and adjacent non-tumor tissue. Receiver-operating characteristic（ROC） curves were constructed, and the area under the ROC curves（AUC）was calculated to evaluate the significance of miR-222 in distinguishing glioma tumor tissues and adjacent normal tissues.Survival curves of Kaplan-Meier method were constructed for both high expression group and low expression group, and the difference between curves was evaluated by log-rank test. Results The significance of miR-222 expression level in distinguishing glioma tumor from adjacent non-tumor tissues was further validated.The expression level of miR-222 was positively correlated with glioma malignancy. Conclusion The expression level of miR-222 in tumor tissues can accurately estimate the prognosis of patients with glioma.