HSP27基因在苏太猪F18大肠杆菌抗性型和敏感型个体间的差异表达分析

Differential Expression of HSP27 Gene between Resistant and Sensitive Individuals to Escherichia coli F18 in Sutai Piglets

热休克蛋白27(heat shock protein 27,HSP27)是哺乳动物普遍存在的小休克蛋白家族成员之一,在参与调解细胞的增殖、分化和细胞凋亡过程中起到了重要的作用。为探讨猪HSP27基因的表达水平及其与F18大肠杆菌抗性的关系,本研究运用荧光定量PCR方法检测HSP27基因在苏太猪F18大肠杆菌病抗性型和敏感型资源群体中各组织的表达水平,并分析在E.coli F18抗性组和敏感组中的表达差异。结果表明:HSP27基因在所有检测个体的11个组织中均有表达,其中肺中表达量最高,其次在肝、脾、肾、十二指肠和空肠中表达较高,而在肌肉、胸腺和淋巴结中表达较低;抗性组和敏感组差异表达显示,在肝脏和淋巴结2个组织中,HSP27基因在抗性组个体中的表达量极显著高于敏感性个体的表达量(P<0.01),在脾脏、胸腺和空肠3个组织中,HSP27基因在抗性组个体中的表达量显著高于敏感性个体的表达量(P<0.05)。HSP27基因在E.coli F18抗性组免疫组织和肠道组织中的高水平表达提示HSP27基因可能在机体抵抗F18大肠杆菌感染过程中发挥了重要的免疫调控作用。

Heat Shock Protein 27 （HSP27） is a member of the small shock protein family widespread in mammals, which plays an important role in mediating cell proliferation, differentiation and apoptosis. To investigate the expression of porcine HSP27 gene and its relationship with the resistance of Escherichia coli FI8, real-time PCR method was used to determine the expression level of HSP27 gene in tissues in resistant and sensitive individuals to Escherichia coli F18 in Sutai pigs, and further analyze the differential expression level between resistant and sensitive individuals to Escherichia coli F18. The results showed that HSP27 genes was expressed in 11 tissues of all detected individuals, which expressed most highly in the lung, followed by the liver, kidney, spleen, duodenum and jejunum. However, it expressed lowly in the muscle, thymus and lymph nodes. The analysis of differential expression showed that the expression level of HSP27 gene in Escherichia coli F18-resistant individuals was very significantly higher than that of Escherichia coll F18-sensitive individuals in the liver and lymph node （P 〈0.01）, and was significantly higher than that of Escherichia coli F18-sensitive individuals in the spleen, thymus and jejunum （P〈0.05）. Higher expression level of HSP27 gene in immune tissues and intestinal tissues of the resistance individuals to Escherichia coli F18 suggests that HSP27 gene may plays an important role in initiating and regulating immune responses in order to improve the resistance to Escherichia coli F18 infection..