微小RNA-126与血管内皮生长因子在胃癌中的表达及意义

Expressions and clinical significances of microRNA-126 and vascular endothelial growth factor in gastric carcinoma

目的观察微小RNA126（miR-126）与血管内皮生长因子（VEGF）在胃癌组织中的表达情况，并探讨其与胃癌临床病理因素的关系。方法收集2012年1月至2014年3月在河南省商丘市第一人民医院就诊的50例胃癌患者的癌组织和癌旁组织，应用反转录聚合酶链反应检测miR-126及VEGF的相对表达水平，分析其相关性并探讨与胃癌相关临床因素的关系。结果miR-126在胃癌组织和癌旁组织的表达量分别为0.652±0.102和0.379±0.069；VEGF在胃癌组织和癌旁组织的表达量分别为1.523±0.142和0.604±0.152，差异均有统计学意义（t=8.374，P=0.001；t=29.508，P=0.000），且二者表达呈正相关（r=0.735，P=0.001）。miR-126及VEGF的表达与胃癌的TNM分期（t=22.981，P=0.001；t=18.053，P=0.001）、浸润深度（t=26.792，P=0.001；t=20.234，P=0.001）及淋巴结转移（t=26.668，P=0.001；t=14.857，P=0.001）相关。结论miR-126在胃癌组织中高表达，并且可能通过促进VEGF的表达导致新生血管形成，从而促进肿瘤的发生发展。

ObjectiveTo observe the expressions of microRNA-126 （miR-126） and vascular endothelial growth factor （VEGF） in gastric carcinoma tissues, and their associations with clinicopathologic features in gastric carcinoma. MethodsFifty cases of gastric carcinoma and matched tumor adjacent tissue specimens were collected from January 2012 to March 2014 in Shangqiu First People′s Hospital of He′nan Province. Expression levels of miR-126 and VEGF were examined using reverse transcriptasepolymerase chain reaction. Their correlations and the relationships between them and the clinicopathologic features in gastric carcinoma were analyzed. ResultsThe expression levels of miR-126 in the tumor tissues and in the adjacent tissues were 0.652±0.102 and 0.379±0.069, and the expression levels of VEGF in the two kinds of tissues were 1.523±0.142 and 0.604±0.152, with statistical differences （t=8.374, P=0.001; t=29.508, P=0.000）. The expressions of miR-126 and VEGF were significantly positively correlated （r=0.735, P=0.001）. The expressions of miR-126 and VEGF were associated with TNM stage （t=22.981, P=0.001; t=18.053, P=0.001）, depth of tumor invasion （t=26.792, P=0.001; t=20.234, P=0.001） and tumor node metastasis （t=26.668, P=0.001; t=14.857, P=0.001）. ConclusionmiR-126 is high expressed in the gastric carcinoma tissue, which may upregulate the expression of VEGF, and lead to the formation of new blood vessels, then to promote gastric carcinogenesis and the development process.