中药益心康对感染CVB_3病毒的新生乳鼠体外心肌细胞凋亡及相关因子Bcl-2/Bax表达的影响

Effect of yixinkang soup on the apoptosis of cardiomyocytes and the expression of related gene Bcl-2/Bax in the newborn suckling rats infected by CVB_3 virus

目的研究中药益心康对感染柯萨奇病毒嗜心肌毒株3型(Coxsackie virus B,CVB3)新生乳鼠体外心肌细胞凋亡及相关基因Bcl-2/Bax表达的影响,探讨中药益心康治疗病毒性心肌炎的作用机制。方法原代培养SD大鼠乳鼠体外心肌细胞,建立感染CVB3的体外心肌细胞模型,将乳鼠心肌细胞分为4组:正常组、病毒组、益心康组、利巴韦林组。采用倒置显微镜观察心肌细胞生长情况及病变程度(CPE),用RT-PCR法检测凋亡相关因子Bcl-2、Bax的表达。结果益心康组给药48 h后细胞CPE略轻于利巴韦林。益心康组Bcl-2 mRNA的相对表达量高于病毒组(P<0.01)及利巴韦林组(P<0.05),而Bax mRNA的相对表达量低于病毒对照组(P<0.01)及利巴韦林组(P<0.05),且Bcl-2/Bax高于病毒组及利巴韦林组(P<0.05)。结论中药益心康能减轻心肌损伤,保护乳鼠心肌细胞,其作用机制与下调促凋亡基因Bax、上调抑制凋亡基因Bcl-2、升高Bcl-2/Bax比值有关。

Objective To study the effect of yixinkang soup on the apoptosis of cardiomyocytes and the expression of related gene Bcl-2 / Bax of newborn suckling rats infected by CVB3 virus,and explore the mechanism. Methods The original generation of SD rat cardiomyocytes were cultivated,and CVB3 infection cardiomyocyte model was established. The cardiomyocyte were divided into 4 groups： normal group,virus group,yixinkang group and ribavirin group. The apoptosis of cardiomyocytes was observed using flowinstrument,and the expression of apoptosis related genes Bcl-2 and Bax was observed by RT-PCR method. Results After 48 h,CPE of yixinkang group was slightly lighter than ribavirin group. The expression of Bcl-2 mRNA in yixinkang group was higher than virus group（ P〈0. 01） and ribavirin group（ P〈0. 05）. The expression of Bax mRNA in yixinkang group was lower than virus group（ P〈0. 01） and ribavirin group（ P〈0. 05）,and Bcl-2 / Bax was higher than virus group and ribavirin group（ P〈0. 05）. Conclusion Yixinkang can alleviate myocardial injury,it has protective effects on the rats＇ cardiomyocytes. The mechanism of action is related to the down-regulation of Bax,up-regulation of Bcl-2,and the increase of Bcl-2 / Bax.