摘要
目的探讨雷公藤甲素对三硝基苯磺酸钠(TNBS)诱导的大鼠结肠炎的疗效,以及对炎性细胞因子IL-4、IL-13表达的影响。方法成年SD大鼠18只,随机分为正常对照组(C组)、TNBS模型组(D1组)、雷公藤甲素组(TP组)。观察大鼠的一般情况,比较结肠组织炎症程度。HE染色观察大鼠结肠组织形态学变化。用反转录聚合酶链反应(RT-PCR)和Western blot(WB)法检测大鼠结肠组织IL-4和IL-13的表达水平。结果PCR和WB法检测结果表明,C组结肠组织IL-4、IL-13的表达水平最高,D1组表达最低,TP组介于两者之间。结论雷公藤甲素能有效治疗TNBS诱导的大鼠结肠炎,其作用机制可能与雷公藤甲素上调大鼠结肠黏膜抑炎因子IL-4、IL-13的表达,抑制炎症的发生发展有关。
Objective To evaluate the curative effect of triptolide on TNBS induced colitis and the expression of intestinal mucosa IL-4 and IL-13 in experimental colitis rats and to investigate the underlying mechanisms. Methods18 adult Sprague-Dawley rats were randomized into three groups： normal control group（ group C）,model group（ group D1）,tripotlide treatment group（ group TP）. The general status of the rats was observed,and the histopathologic changes in the colon were examined. Reverse transcription-polymerase chain reaction（ PCR） and Western blot（ WB）techniques were used to detect the expression of IL-4 and IL-13. Results PCR and WB methods showed that the expression levels of IL-4 and IL-13 in group C were higher than those of group D1 and group TP（ P〈0. 05）,and the expression levels in group D1 were lover than those of group TP（ P〈0. 05）. Conclusion Triptolide could effectively improve the TNBS-induced colitis in rats by promoting the expression of IL-4 and IL-13 and inhibiting the development of inflammation.
出处
《实用药物与临床》
CAS
2015年第11期1284-1288,共5页
Practical Pharmacy and Clinical Remedies
基金
辽宁省自然科学基金课题(201102106)
