雷公藤甲素对MIA模型大鼠膝骨关节炎c-Jun、MMP-9表达及血清炎性标志物的影响

Effect of triptolide on c-Jun,MMP-9 expression and serum inflammatory markers in rat MIA osteoarthritis model

目的观察雷公藤甲素对膝部骨关节炎滑膜c-Jun、MMP-9及血清PGE2、IL-8水平的影响,探讨其在膝部骨关节炎中的药理学价值。方法 45只SD大鼠随机分为正常对照组、模型对照组及雷公藤甲素组,建立碘乙酸钠(Monosodium iodoacetate,MIA)膝关节炎模型,并给予雷公藤甲素干预,以Western blot及(qRT)-PCR法检测c-Jun及M M P-9在膝关节滑膜中蛋白及mRNA水平表达,以酶联免疫法检测外周血中PGE2及IL-8水平。结果 Western blot及(qRT)-PCR显示,c-Jun及MMP-9在膝关节滑膜组织中蛋白及mRNA水平上均有表达,雷公藤甲素组及正常对照组明显低于模型对照组(P<0.01),雷公藤甲素组与正常对照组比较差异无统计学意义(P>0.05);在血清PGE2及IL-8表达上,雷公藤甲素组及正常对照组明显低于模型对照组(P<0.05)。结论雷公藤甲素可抑制骨关节炎滑膜中c-Jun及MMP-9表达,降低外周血PGE2及IL-8水平,抑制炎症反应,对骨关节炎有治疗作用。

Objective To detect the effect of triptolide on c-Jun and MMP-9 in knee osteoarthritis synovium and serum PGE2 and IL-8,discuss the pharmacological value of triptolide. Methods Forty-five SD rats were divided into normal control group,model control group and triptolide group randomly,MIA osteoarthritis models were constructed,and triptolide was given. The c-Jun and MMP-9 expression at protein and mRNA level in knee synovium were detected by Western blot and（ qRT）-PCR. Results Western blot and（ qRT）-PCR showed that c-Jun and MMP-9 were expressed at protein and mRNA levels in synovium tissues,the expression in triptolide group and normal control group was higher than those of model control group（ P〈0. 01）,there was no significant difference between triptolide group and normal control group（ P〉0. 05）. Serum PGE2 and IL-8 in triptolide group and normal control group were lower than those of model control group（ P〈0. 01）,there was no significant difference between triptolide group and normal control group（ P〉0. 05）. Conclusion Triptolide can inhibit c-Jun and MMP-9 expression in osteoarthritis synovium,decrease the PGE2 and IL-8 levels in peripheral blood,inhibit the inflammatory reaction,it has therapeutic effect on osteoarthritis.