电脉冲介导基因转移法在真核细胞表达CD_4和CD_(19)抗原

Electroporation transfer of human leukocyte differentiation antigenes CD_4, CD_(19) genes into mammalian cells

应用电脉冲介导基因转移法,将人白细胞分化抗原CD_4、CD_(19)。基因转入Cos细胞,间接免疫荧光及APAA(?)。联酶标染色表明CD_4和CD_(19)。基因获得高效转移和表达。这为进一步研究其分子结构,功能及筛选、鉴定相应的单克隆抗体提供了新的途径。

Human leukocyte differentiation antigens CD4 and CD19 gene were transfected into Cos cell line by electroporation method. Indirect immunoflucence and APAAP immunocytochemical staining shows that transient expression and highefficiency transformation of CD4, CD19 genes was obtained. It can provide a new way for reserch on molecular strcture and function of IL-2 receptor gene, identification and screen of Anti-IL-2 receptor McABs.