水涝胁迫下欧李丙二醛和氧自由基的变化

Changes of Malondialdehyde and Oxygen Free Radicals in Prunus humilis Bunge Leaves under Waterlogging Stess

对水涝胁迫下欧李丙二醛和氧自由基含量变化进行初步研究,以期获得欧李植物在成都地区的生长情况,为探讨欧李植物的抗逆性提供理论基础。以双组份分光度法测定丙二醛的含量,提取液与TBA在沸水浴中反应后,分别测定450,532和600 nm波长下的吸光度值;利用O-2.与羟胺反应生成NO2-,NO2-在对氨基苯磺酸和a-萘胺作用下,生成粉红色的偶氮染料,检测植物叶片中O-2.含量。结果显示,水涝胁迫下欧李生长期中,丙二醛含量呈上升趋势;水涝胁迫下欧李氧自由基含量也增加。研究认为随水涝胁迫程度的增加,欧李内氧自由基含量增加,氧化脂膜,导致丙二醛含量增加。

MDA and oxygen free radicals were determined to obtain the growing state of Prunus humilis Bunge under waterlogging stress and provide theoretical basis for stress tolerance of Prunus humilis Bunge in Chengdu. The content of MDA was detected by two-component spectrophotometry method. The absorbance at 450, 532 and 600 nm were determined after the extract and TBA reacted in the boiling water bath; the content of O2^-.was detected through pink azo dyestuffs which were obtained by the reaction of O2^-.and hydroxylamine to gain NO2^-, NO2^-then reacted with sulfanilic acid and a-naphthalene amine. The results showed that the content of MDA and O2^-.were all increased during the waterloggin. The contents of oxygen free radicals increased and caused bilayer lipid membrane oxidation; the contents of MDA also increased during the waterlogging because MDA was the product of bilayer lipid membrane oxidation.