摘要
利用叶绿素荧光仪对化学诱变剂乙酰甲基磺酸(EMS)诱变的野生型拟南芥(Col-0生态型)F2代幼苗进行叶绿素荧光突变体筛选,获得了一株低光合效率突变体lpe1(low photosynthetic efficiency 1).遗传分析结果表明,lpe1是一个单基因隐性突变体.在生长发育的各个时期,lpe1叶片的叶绿素荧光值、叶绿素含量、淀粉含量和总蛋白含量明显低于野生型.
The F2 population mutagenized by ethyl methanesulfonate（EMS）was screened by the chloroplast fluorescence imager and a low photosynthetic efficiency mutant lpe1 was screened in Arabidopsis thaliana（ecotype Col-0）.Results of genetic analysis and physiological experiments showed that lpe1 is a single gene recessive mutant.Chlorophyll fluorescence,chlorophyll content,total protein content and starch content of lpe1 leaves were lower than those of the wild type at different growth stages.
出处
《河南大学学报（自然科学版）》
CAS
2015年第6期724-727,共4页
Journal of Henan University:Natural Science
基金
国家自然科学基金项目(U1404303)
河南省基础与前沿技术研究项目(112300410219))
河南省教育厅自然科学研究项目(2011A180023)
关键词
拟南芥
光合效率
突变体
筛选
遗传分析
生理分析
Arabidopsis thaliana
photosynthetic efficiency
mutant
screen
genetic analysis
physiological analysis
