HPLC法同时测定少腹逐瘀丸中芍药苷、阿魏酸、桂皮醛和异鼠李素-3-0-新橙皮苷含量

HPLC Simultaneous Determination of Paeoniflorin,Ferulic Acid,Cinnamaldehyde and Isorhamnetin-3-0-neohespeidoside in Shaofu zhuyu Pills

目的：采用 HPLC 一个条件同时测定少腹逐瘀丸中赤芍、当归、川芎、肉桂和蒲黄中指标成分芍药苷、阿魏酸、桂皮醛和异鼠李素-3-0-新橙皮苷的含量。方法：采用高效液相色谱法，色谱柱为自装 C18柱（富士填料，5μm，4．6 mm x250 mm）；流动相采用梯度洗脱（A 为乙腈，B 为0．1%磷酸溶液），流速：1．0 mL·min -1；柱温：室温；检测波长280 nm，进样量10μL。结果：芍药苷在0．8088-6．0660μg范围内，峰面积与进样量线性关系良好（r2=0．999），平均回收率为97．9%，RSD 为2．5%；阿魏酸在0．0625-0．6252μg范围内，峰面积值与进样量线性关系良好（r2=0．999），平均回收率为99．5%，RSD 为2．7%；异鼠李素-3-0-新橙皮苷在0．11-1．68μg范围内，峰面积值与进样量线性关系良好（r2=0．999），平均回收率为99．2%，RSD 为1．9%；桂皮醛在0．05-0．96μg范围内，峰面积值与进样量线性关系良好（r2=0．999），平均回收率为99．5%，RSD 为1．7%。精密度和重现性 RSD 均小于2．0%。结论：该方法简便、灵敏、准确，分离效果好，可用于少腹逐瘀丸的质量控制。

Objective：To determine the content of four major compents（paeoniflorin,ferulicacid,cinnamalde-hyde and isorhamnetin-3-0-neohespeidoside）in shaofu zhuyu pills by HPLC. Methods：By high performance liquid chromatography（ HPLC） determination,the condition of chromatography was C18（ fuji fillers,4. 6 mm x250 mm;5 μm）. The mobile phase consisted of 0. 1% phosphoric acid and acetonitrile as gradient elute. The flow rate was 1. 0 mL/ min;the column temperature was room temperature;the detection wavelength was 280 nm. Injection volume was 10 μL. Results：Paeoniflorin showed a good linear relationship（r2 = 0. 999）at a range of 0. 8808 - 6. 066μg,with the average recoverery 97. 9% ,and RSD was 2. 5% . Ferulic acid showed a good linear relationship（r2 = 0. 999）at a range of 0. 062 52 - 0. 625 2 μg,with the average recoverery 99. 5% ,and RSD was 2. 7% . Cinnamaldehyde showed a good linear relationship（r2 = 0. 999）at a range of 0. 048 - 0. 96 μg,with the average recoverery 99. 5% ,and RSD was 1. 7% . Isorhamnetin-3-0-neohespeidoside showed a good linear relationship（r2 = 0. 999） at a range of 0. 112 -1. 68 μg,with the average recoverery 99. 2% ,and RSD was 1. 9% . The precision and reappearance of the method were less than 2. 0% . Conclusion：The method is simple,precise and reproducible,having a good separation effect,which can be used for the quality control of shaofu zhuyu pills.