浙江省中东部白纹伊蚊共生菌沃尔巴克氏体的调查研究

Investigation on symbiotic bacteria(Wolbachia) in Aedes albopictus from central and eastern Zhejiang province, China

目的了解浙江省中东部地区白纹伊蚊种群中沃尔巴克氏体（Wolbachia）的感染状况及其基因型别,为蚊虫和蚊媒传染病的生物防制提供依据。方法用诱蚊灯法和人诱法采集浙江省中东部地区的白纹伊蚊雌性成蚊,PCR扩增沃尔巴克氏体的wsp基因,测序并进行系统进化分析。结果在6个白纹伊蚊采样点（杭州市、宁波市、嵊泗县、义乌市、丽水市和温州市）捕获白纹伊蚊雌性成蚊共192只,各个白纹伊蚊种群的沃尔巴克氏体核酸阳性率在25.0%~96.9%之间。获得11个wsp单倍型序列,序列长度在581~605 bp之间,碱基A、T、G、C的平均含量分别为31.4%、31.1%、21.5%和16.0%。各个wsp核酸序列的同源性在76.5%~99.8%之间,对应的氨基酸序列同源性在66.2%~100%之间。系统进化分析结果显示,11个单倍型序列被划分在2个超级组别（A组和B组）中,超级组别A属于w Alb A型沃尔巴克氏体,超级组别B属于w Alb B型沃尔巴克氏体。结论浙江省中东部白纹伊蚊普遍感染沃尔巴克氏体,其基因型为wAlbA和wAlbB型。

Objective To investigate the infection state and genotype of Wolbachia from Aedes albopictus in central and eastern Zhejiang province, and to provide a reference for mosquito and mosquito-borne disease control.Methods Light traps and human baits were applied to collect adult female mosquitoes, wsp genes of Wolbachia were tested by PCR and sequenced for phylogenetic analysis.Results In total 192 adult female mosquitoes were captured in six sampling sites：Hangzhou, Ningbo, Shengsi, Yiwu, Lishui and Wenzhou. The positive rate of wsp gene of Wolbachia was between 25.0%and 96.9% among these cities. There were 11 wsp haplotype sequences, of which lengths were between 581 bp and 605 bp,the average content of A, T, G and C was 31.4%, 31.1%, 21.5%, 16.0%, respectively. The homology of the nucleotide sequences of wsp was between 76.5% and 99.8%, and the homology of the amino acid sequences of wsp was between 66.2%and 100%. The 11 wsp haplotype sequences were divided in super group A（Wolbachia： w Alb A） and B（Wolbachia： w Alb B）by phylogenetic analysis.Conclusion Infection of Wolbachia in Ae. albopictus mosquitoes are widespread by in centraland eastern Zhejiang province, whose genotypes belong to w AlbA and w AlbB.