广东省蚊媒西尼罗病毒携带情况调查研究

Investigation of West Nile virus carried by mosquito vectors in Guangdong province

目的了解广东省蚊媒的构成、发生消长及西尼罗病毒的携带情况,为及时有效地切断西尼罗病毒病传播途径提供科学依据。方法 2013年5月至2014年4月在广东省的广州、佛山、东莞、茂名市的城区和郊区设点,采用灯诱法调查成蚊;一步法反转录-聚合酶链反应(RT-PCR)技术检测蚊媒携带的西尼罗病毒。结果广东省蚊媒平均密度为11.80只/灯,其密度高峰在4-5月,生境中以牲畜棚中的密度最高。致乏库蚊/致倦库蚊占捕蚊总数的89.90%,为优势蚊种。RT-PCR法未检测到西尼罗病毒阳性。结论致乏库蚊/致倦库蚊是广东省的优势蚊种,加强监测可以掌握其消长规律,为制定适宜的防制措施和预报预警其可能传播的西尼罗病毒提供科学依据;对广东省内西尼罗热的传播媒介种类及其传播流行风险有待进一步研究。

Objective To study the community structure, dynamic and West Nile virus（WNV） carried of mosquito vectors in Guangdong, and provide scientific evidence for further development of control and prevention strategies.Methods Mosquito density was monitored by Light-trap from May 2013 to April 2014. One-step reverse-transcript PCR was applied.Results The average density of mosquito was 11.80/light. The seasonal peak of density was from May to July. Of all habitats the density in the barn was the highest. Culex pipiens quinquefasciatus accounts for 89.90%, the largest proportionof mosquitoes. No positive was found by RT- PCR.Conclusion Culex pipiens quinquefasciatus was the predominantspecies, the monitoring data could provide scientific basis for the control strategies and warning and forecasting of WNV,but the vectors species of West Nile fever in Guangdong province and its spread risk needs to be further studied.