血清类黏蛋白1样蛋白3表达与5岁以下儿童喘息的相关性分析

Correlation between expression of orosomucoid 1-like 3 and wheezing of children under 5 years old

目的探讨血清类黏蛋白1样蛋白3(ORMDL3)基因表达量与5岁以下儿童喘息的相关性。方法选取2013年8月—2014年12月在上海交通大学医学院附属仁济医院南院儿科就诊的89例5岁以下喘息患儿,依据哮喘预测指数(API)严格标准分为API^+(过去1年内喘息次数≥4)组(n=42)和API-(过去1年内喘息次数1~3次)组(n=47);另设API0(5岁以下健康儿童)对照组(n=51)。检测3组血清ORMDL3基因表达量,分析血清ORMDL3表达水平与API、喘息、鼻炎、总免疫球蛋白E(TIg E)、家族史、湿疹、年龄、尘螨等吸入性过敏原及牛奶等摄入性过敏原的相关性。结果 API^+组患者血清ORMDL3表达水平显著高于API-和API0组。ORMDL3表达水平与API(r=0.405,P=0.000)、喘息(r=0.492,P=0.000)、湿疹(r=0.454,P=0.000)、户尘螨(r=0.298,P=0.000)、狗毛皮屑(r=0.251,P=0.000)和TIg E(r=0.170,P=0.002)存在正相关;与性别、家族史、鼻炎、年龄、屋尘螨、猫毛皮屑、树木、点青/分枝/烟曲/黑曲/交链霉及饮食因素(牛奶、鸡蛋白、牛肉、腰果、蟹、虾)不存在相关性(P>0.05)。结论 ORMDL3基因表达量与儿童API之间呈正相关,提示ORMDL3表达量与API相结合可作为诊断5岁以下儿童哮喘的诊断依据之一。

Objective To investigate the correlation between the gene expressive quantity of orosomucoid 1-like3（0RMDL3） and wheezing of children under 5 years old.Methods A total of 89 children with wheezing who were under 5 years old and treated at the Department of Pediatric of Renji Hospital South Campus affiliated to Shanghai Jiao Tong University School of Medicine from August 2013 to December 2014 were enrolled.According to the strict criteria of asthma predictive index（API）,children were divided into API~＋ group（n=42）（the number of onsets of asthma ≥4 in the past year） and API^- group（n=47）（the number of onsets of asthma between 1 and 3 in the past year）.Health children aged less than 5 years old were selected as controls（APIO group,n = 51）.The gene expressive quantity of ORMDL3 of three groups was detected.The correlations of the expression level of serum ORMDL3 and API,wheezing,rhinitis,total IgE,family history,eczema,age,inhalation allergens such as dust mite,and intake allergens such as milk were analyzed.Results The expression level of serum ORMDL3 of API~＋ group was significantly higher than that of API^- group and APIO group.The expression level of ORMDL3 was positively correlated with API（r =0.405,P = 0.000）,wheezing0=0.492,P = 0.000）,eczema（r =0.454,P = 0.000）,house dust mite（r =0.298,P = 0.000）,dog hair and scurf（r =0.251,P = 0.000）,and total IgE（r =0.170,P = 0.002）.The expression level of 0RMDL3 was not correlated with sex,family history,rhinitis,age,dermatophagoides,cat hair and scurf,tree,penicillium notatum,Piptocephalis,aspergillus fumigatus,alternaria,and dietary factors（milk,egg white,beef,cashew nut,crab,shrimp）（P〉0.05）.Conclusion The gene expressive quantity of 0RMDL3 positively correlates with API of children,which suggests that combination of the gene expressive quantity of 0RMDL3 and API can be regarded as one of evidences for the diagnosis of asthma for children under 5 years old.