姜黄素缓解2型糖尿病小鼠肝脏的代谢紊乱

Benefits of Curcumin on Hepatic Metabolic Disorders in Type 2 Diabetic Mice

目的:研究姜黄素(curcumin)对2型糖尿病(type 2 diabetes mellitus,T2DM)小鼠肝脏代谢紊乱的影响。方法:建立T2DM小鼠模型,将造模成功的T2DM小鼠随机分成姜黄素给药组(50 mg/kg、100 mg/kg)和模型组,正常小鼠作为阴性对照组。通过生化指标检测、酶联免疫吸附、免疫印迹、和苏木素-伊红染色实验,探讨姜黄素对糖尿病肝脏的药理活性。结果:同糖尿病组相比,姜黄素能够改善血清和肝脏甘油三酯、总胆固醇、低密度脂蛋白、谷草谷丙转氨酶等生化指标(P<0.05)。研究发现,姜黄素降低糖尿病小鼠肝脏糖皮质激素水平(P<0.05),并伴随着糖皮质激素受体蛋白表达明显降低,显著性低于糖尿病模型组(1.17±0.23 vs Cur 100 mg/kg0.40±0.20,P<0.01)。病理切片确证姜黄素缓解糖尿病肝脏脂肪变性。结论:姜黄素具有调节糖尿病小鼠代谢紊乱的药理活性,可能是通过缓解糖皮质激素信号通路紊乱的作用。

Objective： To investigate the effects of curcumin on hepatic metabolic disorders in type 2 diabetic（ T2DM）mice. Methods： T2 DM mice were established as previous method,and then were randomly divided into curcumin-treated group（ 50 mg / kg,100 mg / kg） and vehicle-treated DM group. The normal mice were considered as control group.Through automatic analysis,enzyme linked immunosorbent assay,western blot and hematoxylin-eosin staining,the present study explained the pharmacological effects of curcumin. Results： Compared with diabetic mice,treatment with curcumin significantly decreased the levels of triglyceride,total cholesterol and low density lipoprotein in serum（ P 〈0. 05） and inactivated the activities of glutamic-oxalacetic transaminase and glutamic-pyruvic transaminase in liver tissues（ P 〈0. 05）. Curcumin also reduced the expression of GR protein to relative 0. 40 ± 0. 20,which was less than relative 1. 17 ±0. 23 in diabetic mice（ P 〈0. 01）. Furthermore,histopathological analysis demonstrated that curcumin could improve the structural abnormalities in diabetic liver tissues. Conclusions： Curcumin has the ability of alleviating metabolic disorders in T2 DM mice,which may depend on effectively attenuating diabetes-induced glucocorticoid excess.