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紫外可见分光光度法测定鲫鱼胆中总黄酮的含量 被引量:1

Determination of Total Flavone in Jasminum nervosum Lour. by UV-VIS Spectrophotometry
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摘要 目的:测定鲫鱼胆中总黄酮的含量。方法:以芦丁为对照品,NaNO_2-Al(NO_3)_3为显色剂,采用紫外可见分光光度法测定,检测波长为507 nm。结果:样品中总黄酮在浓度7.08~141.6 mg·L^(-1)范围内呈良好线性关系,回归方程为Y=11.223X-0.001(r=0.999 3),线性良好,加样回收率为101.73%,RSD为2.67%。结论:该方法简便、灵敏、准确,可用于鲫鱼胆中总黄酮的含量测定。 Objective: To determine the content of total flavone in Jasminum nervosum Lour. Methods: Rutin and NaNO_2-Al(NO_3)_3 system were employed as reference substance and chromogenic reagent,respectively. The total flavonoids of Jasminum nervosum Lour. were measured by UV-VIS spectrophotometry at the 507 nm. Results : Linear relation of the method was good. The regression equation was Y = 11. 223X-0. 001( r = 0. 999 3) and the average recovery rate was 101. 73% and RSD was 2. 67%. Conclusion: This method is simple,sensitive and accurate and can be used for determination the content of flavonoids in Jasminum nervosum Lour.
作者 周倩 滕建北
机构地区 广西中医药大学
出处 《中华中医药学刊》 CAS 北大核心 2015年第12期2951-2954,共4页 Chinese Archives of Traditional Chinese Medicine
基金 广西自然科学基金创新研究团队项目(2011GXNSFF018006)
关键词 鲫鱼胆 总黄酮 含量测定 紫外-可见分光光度法 Jasminum nervosum Lour. flavonoids content determination UV-VIS spectrophotometry
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