摘要
目的:测定鲫鱼胆中总黄酮的含量。方法:以芦丁为对照品,NaNO_2-Al(NO_3)_3为显色剂,采用紫外可见分光光度法测定,检测波长为507 nm。结果:样品中总黄酮在浓度7.08~141.6 mg·L^(-1)范围内呈良好线性关系,回归方程为Y=11.223X-0.001(r=0.999 3),线性良好,加样回收率为101.73%,RSD为2.67%。结论:该方法简便、灵敏、准确,可用于鲫鱼胆中总黄酮的含量测定。
Objective： To determine the content of total flavone in Jasminum nervosum Lour. Methods： Rutin and NaNO_2-Al（NO_3）_3 system were employed as reference substance and chromogenic reagent,respectively. The total flavonoids of Jasminum nervosum Lour. were measured by UV-VIS spectrophotometry at the 507 nm. Results ： Linear relation of the method was good. The regression equation was Y = 11. 223X-0. 001（ r = 0. 999 3） and the average recovery rate was 101. 73% and RSD was 2. 67%. Conclusion： This method is simple,sensitive and accurate and can be used for determination the content of flavonoids in Jasminum nervosum Lour.
出处
《中华中医药学刊》
CAS
北大核心
2015年第12期2951-2954,共4页
Chinese Archives of Traditional Chinese Medicine
基金
广西自然科学基金创新研究团队项目(2011GXNSFF018006)
关键词
鲫鱼胆
总黄酮
含量测定
紫外-可见分光光度法
Jasminum nervosum Lour.
flavonoids
content determination
UV-VIS spectrophotometry
