Th17细胞相关因子在子痫前期和正常妊娠孕妇外周血和胎盘中表达量的对比

Comparison of expression levels of Th17-associated factors in placenta and peripheral blood between women with pre-eclampsia and healthy women

目的通过对子痫前期及正常妊娠的孕妇外周血及胎盘组织中Th17细胞相关因子IL-17、IL-21、IL-22、HLA-G及RORc表达量的检测以及miRNA-21相对表达量的比较,探讨Th17细胞相关因子以及miRNA-21在子痫前期发病中的作用。方法选取2012年3月至2014年5月镇江妇幼保健院的40例孕妇为研究对象,其中子痫前期孕妇20例,正常孕妇20例,采用流式细胞仪分别检测子痫前期组和正常对照组外周血中Th17细胞的水平,采用实时定量PCR法检测外周血和胎盘组织中Th17细胞相关细胞因子IL-17、IL-21、IL-22、HLA-G、RORc的表达量和相对表达量,采用实时定量PCR法检测胎盘组织中miRNA-21的相对表达量。结果初步证实子痫前期患者和正常妊娠女性外周血Th17细胞相关细胞因子IL-17、IL-21、IL-22、HLA-G、RORc水平之间存在差异,子痫前期患者和正常妊娠女性胎盘组织中Th17细胞相关细胞因子IL-17、IL-21、IL-22、RORc、HLA-G水平存在差异。(1)子痫前期患者的IL-17、IL-21、IL-22的相对表达量明显高于正常对照组,差异有统计学意义(P<0.05)。(2)子痫前期组的HLA-G、RORc的相对表达量明显低于正常对照组,差异有统计学意义(P<0.05)。(3)子痫前期组的miRNA-21的相对表达量明显高于正常对照组,差异有统计学意义(P<0.05)。(4)各项指标之间及各检测指标与临床指标间存在相关性。结论 Th17细胞相关细胞因子IL-17、IL-21、IL-22、HLA-G、RORc水平在子痫前期的发病过程中可能发挥重要作用,通过对相关指标的监测可能预测子痫前期的发生和监测病情变化,阻断这一途径就有可能有效预防和治疗子痫前期。

Objective To study the relationship between Th17 cells and relative factors with pregnancy-induced hypertension disease, we compared the expression of relative factors of Th17 cells in placenta and peripheral blood from women with pre-eclampsia and healthy women. Methods This case-control study was conducted on 40 pregnant women. This comprised 20 women with pre-eclampsia and 20 healthy, age-matched pregnant women （controls）. Expression of IL-17, IL-21, RORc, HLA-G and miRNA-21 in placenta and peripheral blood were measured by flow cytometry （FCM）, ELISA and polymerase chain reaction （PCR）. Results It was confirmed that patients with pre-eclampsia and normal pregnancy women showed different expressions of IL-17, IL-21, RORc and HLA-G associated with Th17 cells in peripheral blood and placenta as follows. （1） Expressions of IL-17 and IL-21 in patients with pre-eclampsia were higher than those in healthy women. The difference was statistically significant （P＆lt; 0.05）. （2） Expressions of RORc and HLA-G in patients with pre-eclampsia were lower than those in healthy women. The difference was statistically significant （P＆lt; 0.05）. （3） Expression of miRNA-21 in patients with pre-eclampsia was higher than that in healthy women. The difference was statistically significant （P＆lt; 0.05）. （4） These data have correlation with clinical indicators. The difference was statistically significant （P＆lt; 0.05）. Conclusion Expressions of IL-17, IL-21, RORc and HLA-G associated with Th17 cells play an important role in pre-eclampsia. Through monitoring relevant indicators, the occurrence of pre-eclampsia might be predicted and monitored for the change of condition. Through early diagnosis and intervention along with safe and effective treatment, the quality of patient lives with pre-eclampsia could be greatly improved, thus saving more medical resources.