基因重组肿瘤坏死因子对LAK细胞体内外抗肿瘤作用的增强效果

Enhancing effect of tumor necrosis factor (TNF) on the in vitro and in vivo antitumor activity of LAK cells

TNF单独不能诱导LAK活性,也不能进一步提高最适剂量IL-2(1000U/ml)诱导的LAK活性,但能显著增强亚适剂量IL-2(10～100U/ml)诱导的LAK活性。抗TNF单抗和抗IL-2受体β链(p75)单抗显著抑制TNF/IL-2对LAK活性的协同诱导作用。实验证明,TNF能显著增强IL-2/LAK细胞的体内抗肿瘤效果,对于腹水型肝癌小鼠,以联合腹腔内注射TNF/IL-2/LAK细胞的体内抗肿瘤效果最佳。

Although TNF alone was not capable of inducing LAK activity and could not enhance the LAK activity induced by IL-2 at optimal concentration (1000U/ml), TNF (500U/ml) was found to act synergistically with IL-2 at suboptimal concentration (10～100U/ml). This TNF/IL-2 synergistic effect was blocked by anti-TNF McAb (Z8) or anti-IL-2Rβ chain McAb (TU27). When TNF and IL-2/LAK cells were combined to treat tumor-bearing mice,TNF could potentiate significantly the therapeutic effect of IL-2/LAK cells in vivo.