RARRES3和MIEN1蛋白在非小细胞肺癌中的表达及其意义

Expression and significance of RARRES3 and MIEN1 protein in non-small cell lung cancer

目的探讨视黄酸受体3(RARRES3)和迁移侵袭增强因子1(MIEN1)在非小细胞肺癌(NSCLC)组织中的表达水平及两者与其临床病理特征和预后的关系。方法收集在本院行手术切除的86例NSCLC组织及72例对应癌旁组织,采用免疫组化SP法分别检测癌组织及癌旁组织中RARRES3和MIEN1的表达情况并根据二级计分法将其分为低表达和高表达部分,分析两蛋白表达水平与临床病理参数(性别、年龄、病理类型、肿瘤大小、分化类型、TNM分期、T分期、吸烟史和淋巴结转移)的关系及两者表达的相关性。结果 RARRES3和MIEN1的阳性颗粒均位于细胞质中,且NSCLC组织RARRES3以低表达为主,其高表达率为29.07%(25/86),低于癌旁组织的55.56%(40/72),NSCLC组织MIEN1以高表达为主,其高表达率为62.79%(54/86),高于癌旁组织的31.94%(23/72),差异均有统计学意义(P<0.05);NSCLC组织中RARRES3和MIEN1蛋白表达均与TNM分期和淋巴结转移有关,RARRES3蛋白亦与肿瘤大小有关,MIEN1蛋白与分化类型有关(P<0.05);RARRES3和MIEN1蛋白表达呈负相关(r=-0.411,P=0.000)。组织RARRES3和MIEN1表达在NSCLC诊断中的AUC、灵敏度和特异度分别为0.825和0.779,85.1%和75.4%,79.2%和83.3%,且RARRES3高表达者的中位总生存期(OS)为32.5个月,高于低表达组的16.0个月,MIEN1高表达者的中位OS为15.5个月,低于低表达组的28.0个月(P<0.05)。结论 RARRES3在NSCLC组织中低表达,而MIEN1在NSCLC组织中高表达,且均与TNM分期和淋巴结转移及预后有关,可能在NSCLC的发生发展中有一定作用。

Objective To investigate the expression levels of retinoic acid receptor 3（ RARRES3） and migration and invasion enhancement factor 1（ MIEN1） in non-small cell lung cancer（ NSCLC） tissues and their relationship with clinical pathological features and prognosis. Methods In this study,86 cases of NSCLC tissues and 72 cases of adjacent tissues were collected from our hospital. The expression of RARRES3 and MIEN1 in cancer tissues and adjacent tissues were detected by immunohistochemical SP method,and the expression level was divided into low expression and high expression level according to the two-grade score. The relationship between the expression level of two protein and clinical pathological parameters（ gender,age,pathological type,tumor size,differentiation type,TNM stage,T stage,smoking history and lymph node metastasis） were analyzed. We further investigated the correlation between both proteins. Results The positive particles of RARRES3 and MIEN1 were located in the cytoplasm. The expression of RARRES3 in NSCLC tissues was 29. 07%（ 25 /86）,lower than 55. 56%（ 40 /72） of para-carcinoma tissues（ P〈0. 05）,while the expression of MIEN1 in NSCLC tissues was 62. 79%（ 54 /86）,higher than 31. 94%（ 23 /72） of para-carcinoma tissues（ P〈0. 05）.The expression of RARRES3 and MIEN1 protein in NSCLC was related to TNM stage and lymph node metastasis. Moreover,RARRES3 was also associated with tumor size,and MIEN1 protein was also associated with differentiation type（ P〈0. 05）. RARRES3 protein expression were negatively correlated with MIEN1（ r =- 0. 411,P = 0. 000）. The AUC,sensitivity and specificity of RARRES3 and MIEN1 in NSCLC were 0. 825 and 0. 779,85. 1% and 75. 4%,79. 2% and 83. 3%,respectively. The median overall survival（ OS）was 32. 5 months in the RARRES3 high expression group,which was higher than 16. 0 months in the low expression group,and the median OS was 15. 5 months in MIEN1 high expression group,which was lower than 28. 0 months in the low expression group（ P〈0. 05）. Conclusion In NSCLC tissues,RARRES3 was lowly expressed and MIEN1 was highly expressed. Both proteins were related to TNM staging,lymph node metastasis and prognosis. It may be related to the occurrence and development of NSCLC.