TLR-9在宫颈鳞癌组织中的表达及其与MVD和VEGF的关系

Expression of TLR-9 receptor in cervical squamous cell carcinoma and its correlation with VEGF and MVD

目的检测宫颈鳞癌组织中Toll样受体-9(TLR-9)表达及其与微血管密度(MVD)和血管内皮生长因子(VEGF)的关系。方法采用免疫组织化学SP法检测48例宫颈鳞癌和20例正常宫颈上皮组织中TLR-9表达,用CD34单克隆抗体标记微血管并计算MVD,同时采用酶联免疫吸附法检测宫颈鳞癌组织中VEGF水平。采用受试者工作特征曲线评价TLR-9表达诊断宫颈鳞癌的效能。结果宫颈鳞癌组织中TLR-9受体的阳性表达率为83.3%(40/48),组织MVD和VEGF水平分别为36.8±7.25和(25.58±3.49)ng/mg,均高于正常宫颈上皮组织(P<0.05);TLR-9受体的高表达与肿瘤的临床分期和基质金属蛋白酶9表达有关(P<0.05),与组织学分级及淋巴结转移无关(P>0.05);TLR-9受体表达阳性者的MVD和VEGF水平明显高于阴性者(P<0.05)。组织TLR-9表达在宫颈鳞癌诊断中的效能较好,其AUC、灵敏度和特异度分别为0.830、82.7%和76.1%。结论 TLR-9高表达可能参与宫颈鳞癌的发生发展及浸润转移,并与宫颈鳞癌的微血管新生密切相关。

Objective To explore the expression of Toll like receptor-9（ TLR-9） and its correlation with microvessel density（ MVD） and vascular endothelial growth factor（ VEGF） in cervical squamous cell carcinoma. Methods The expression of TLR-9 was detected by immunohistochemical SP method in 48 cases of cervical squamous cell carcinoma and 20 cases of normal cervical epithelium. The tissues were labeled with CD34 monoclonal antibody to calculate MVD,and the level of VEGF was measured by enzyme linked immunosorbent assay. The effectiveness of TLR-9 expression in the diagnosis of cervical squamous cell carcinoma was evaluated by receiver operating characteristic curve. Results In the tissues from cervical squamous cell carcinoma,the positive expression rate of TLR-9 was 83. 3%（ 40 /48）,and the MVD and level of VEGF were 36. 8 ± 7. 25 and（ 25. 58 ± 3. 49） ng/mg,all higher than those of normal cervical epithelial tissues（ P〈0. 05）. The high expression of TLR-9 was related to the clinical stage and the expression of matrix metalloproteinase 9,but unrelated with histological grade and lymph node metastasis. The levels of VEGF and MVD in tissues with TLR-9 positive expression were significantly higher than those in the negative group（ P〈0. 05）. The diagnostic efficiency of TLR-9 in cervical squamous cell carcinoma was good,and its AUC,sensitivity and specificity were 0. 830,76. 1% and 82. 7%,respectively. Conclusion The carcinogenesis and metastasis with high expression of TLR-9 may be involved in cervical squamous cell carcinoma,and angiogenesis is closely related with cervical squamous cell carcinoma.