肺腺癌组织中lncRNA CADM1-AS1表达及其临床意义

Expression and clinical significance of lncRNA CADM1-AS1 in lung adenocarcinoma

目的探讨长链非编码lncRNA CADM1-AS1在肺腺癌组织中的表达水平及其临床意义。方法收集于本院行手术切除的72例肺腺癌组织及对应癌旁组织,采用实时定量PCR(q PCR)法检测以上组织标本中的CADM1-AS1水平,分析CADM1-AS1水平与临床病理参数(性别、年龄、肿瘤大小、分化类型、临床分期、T分期、吸烟史和淋巴结转移)的关系,同时根据远期生存资料比较不同CADM1-AS1表达水平的中位总生存期(OS)。进一步采用q PCR法检测常见非小细胞肺癌株A549、SPC-A、NCI-H1650和NCI-H1299中的CADM1-AS1水平。采用受试者工作特征曲线(ROC)评价组织CADM1-AS1水平在肺腺癌早期诊断中的效能。结果肺腺癌组织中的CADM1-AS1水平低于癌旁组织,且与正常支气管上皮细胞株16HBE相比,非小细胞肺癌株的CADM1-AS1均为低表达,差异有统计学意义(P均<0.05),且按照由高至低依次为NCI-H1299>SPCA>NCI-H1650>A549;CADM1-AS1表达与肺腺癌的TNM分期、分化类型、肿瘤大小、T分期及淋巴结转移有关,其中TNMⅡ、Ⅲ期、低未分化、肿瘤最大径>3 cm、T分期T3、T4期及有淋巴结转移组织的CADM1-AS1水平均低于对应项(P均<0.05);CADM1-AS1高水平者的中位OS为32.5个月,高于低水平者的16.0个月,差异有统计学意义(P均<0.05)。组织CADM1-AS1水平在肺腺癌早期诊断中的价值较好,其曲线下面积为0.874,灵敏度和特异度分别为87.8%和85.2%。结论 CADM1-AS1在肺腺癌组织及非小细胞肺癌细胞株中低表达,且与TNM分期、分化类型等临床病理参数及预后有关,可能在肺腺癌的发生发展中有一定作用,同时对于肺腺癌的早期诊断有较好价值。

Objective To explore the expression and clinical significance of lncRNA CADM1-AS1 in lung adenocarcinoma.Methods In this study,72 cases of lung adenocarcinoma tissues and corresponding para carcinoma tissues were collected from our hospital. Real time quantitative PCR（ q PCR） was used to detect the level of CADM1-AS1 in tissue samples. The relationship between CADM1-AS1 level and clinical pathological parameters（ gender,age,tumor size,differentiation,clinical stage,T stage,smoking history and lymph node metastasis） was analyzed. The median overall survival（ OS） was compared between different CADM1-AS1 levels according to the long-term survival data. The levels of CADM1-AS1 in common non-small cell lung cancer cells（ NCI-H1299,SPC-A,NCI-H1650 and A549） were detected by q PCR assay. The effectiveness of CADM1-AS1 level in early diagnosis of lung cancer was evaluated by the receiver operating characteristic curve（ ROC）. Results The level of CADM1-AS1 in lung adenocarcinoma tissues was lower than that in para carcinoma tissues（ P〈0. 05）. Compared with the normal bronchial epithelial cell line 16 HBE,the CADM1-AS1 of the non small cell lung cancer was lower（ P〈0. 05）. CADM1-AS1 level was related to TNM stage,differentiation,tumor size,T stage and lymph node metastasis of lung adenocarcinoma（ P〈0. 05）. The median OS in patients with high CADM1-AS1 level was32. 5 months,higher than 16. 0 months of patients with low CADM1-AS1 level（ P〈0. 05）. The value of CADM1-AS1 in the early diagnosis of lung adenocarcinoma was good with the area under the curve of 0. 874,and the sensitivity and specificity were 87. 8% and85. 2% respectively. Conclusion There were relative low levels of CADM1-AS1 in lung adenocarcinoma cell line and non-small cell lung cancer cell line. The CADM1-AS1 level is related to the TNM stage,differentiation and prognosis,which may be related to the occurrence and development of lung adenocarcinoma. It has a good value for early diagnosis of lung adenocarcinoma.