三维荧光化学多维校正方法研究新进展

Research Progress of Three-dimensional Fluorescence Coupled with Chemical Multiway Calibration

荧光分析是分析科学领域广泛应用的高灵敏度的技术。但常面临单组分测定难以避开干扰、多组分测定困于光谱重叠的难题。化学计量学多维校正方法具有很好的"数学分离分析"功能,通过"数学分离"代替或增强"化学或物理分离"。三维荧光技术结合化学计量学多维校正方法具有独特的"二阶优势",即使有未校正光谱干扰共存,亦能对复杂体系中多组分目标物进行直接、快速、同时、精准的定量分析。目前,该类分析策略正被越来越广泛地应用于医药、生物、食品、环境等领域的复杂测试对象中的多组分目标物的直接、快速、同时定量分析。

Fluorescent technology is widely used in many fields due to its high sensitivity. However,the direct quantification of one target analyte in complex system is still difficult to be achieved when using the traditional fluorescent method without any pretreatment separation procedure. This is due to the fact that serious overlapping of fluorescence spectra often occurs,mainly originating from natural interferences in complex sample backgrounds,or the interferents with similar structures to a target analyte,particularly in the simultaneous analysis of multi-components samples. The rapid development of modern analytical instruments and three-way data collection techniques has led to a resurgence of interest in the development of chemometrics-based analytical strategies,which might light a new avenue to simple experimentation using ＂mathematical separation＂as a replacement or enhancement of ＂physical or chemical separation＂of uncalibrated background or interferents.These methods can offer a highly attractive property,called ＂second-order advantage＂,which allows for the direct and rapid determination of a single target component or simultaneous determination of multiple target components in complex samples,even in the presence of uncalibrated interferences. The property has been a hotspot in the current chemometric domain,and was successfully employed for many applications,such as pharmaceuticals,biological,food,environmental analysis and so on.