暗纹东方鲀卵子磷酸酶等生化指标分析与卵质评估

Analysis of phosphatase and other biochemical parameters of egg and its quality determination in Takifugu obscurus

为了研究暗纹东方鲀卵子可发育能力与其生化指标的关系,用生化分析方法对暗纹东方鲀未受精卵子基本成分、磷酸酶活力、苹果酸脱氢酶（MDH）活力、唾液酸（SA）、氨基酸含量进行分析,同时用GC-MS方法对脂肪酸组成及相对含量进行检测,并对各物质变化规律与其孵化率的相关性进行分析。结果显示,酸性磷酸酶活力（ACP）、SA含量与孵化率显著相关,碱性磷酸酶（AKP）与孵化率显著负相关;C14：0与孵化率显著相关,并呈现递减趋势;ARA、DHA与孵化率均显著相关,并呈现先递增后递减的变化趋势;n-3脂肪酸、DHA与EPA之比、EPA与ARA之比均与孵化率存在显著相关。结果表明,磷酸酶、SA、脂肪酸等物质变化规律与卵子孵化率存在相关性,且ACP活力值在68.045-141.038,AKP活力值在2.298-7.241,SA含量在9.081-19.973,C14：0含量在0.485-0.658,ARA含量在1.371-2.498,DHA含量在13.838-18.469时,均可作为卵子孵化率不低于50%的参考指标,据此可在一定程度上反映暗纹东方鲀苗种繁育生产中所用卵子的可孵化价值,亦可作为参考指标对其卵子质量进行初步的客观评价。

To investigate the relationship between the developmental capacity and biochemical parameters of egg in Takifugu obscurus,the basic components,phosphatase activity,malate dehydrogenase activity,sialic acid,amino acid content in unfertilized eggs of T. obscurus were measured by means of biochemical analysis,also fatty acids composition were measured by means of GC-MS,and the correlation between components and hatching rates were analysed. Results of experiments showed that activity of acid phosphatase,sialic acid content and the hatching rate were significantly correlated,alkaline phosphatase and hatching rate were significantly negative correlated. C14： 0 and hatching rate were significantly negative correlated,and showed a decline trend. ARA,DHA and hatching rates were significantly correlated,and both of them showed a tendency of first increasing and then decreasing with the hatching rate. The content of n-3fatty and the ratio of DHA and EPA and the ratio of EPA and ARA all had significant correlation with the hatching rate. The results demonstrated that phosphatase,sialic acid,fatty acid and egg hatching rate have correlation,also when the activity of ACP were in the range of 68. 045,141. 038,the activity of AKP in the range of 2. 298-7. 241,the content of SA in the range of 9. 081-19. 973,the content of C14： 0 in the range of 0. 485-0. 658,the content of ARA in the range of 1. 371-2. 498,the content of DHA in the range of 13. 838-18. 469,all of them could be the references of which hatching rates were no less than50%. Accordingly these references could reflect the developing value of T. obscurus ＇ egg in breeding production,which could also be used as reference index for embryo and objective evaluation of the quality of its egg.