育真熄风汤对拟帕金森小鼠脑内nNOS和iNOS的影响

Effects of Yuzhenxifeng Decoction on nNOS and iNOS expression in Parkinson model mice

目的探讨中药复方育真熄风汤(YZXFD)对拟帕金森病(PD)模型小鼠黑质内nNOS和iNOS的影响。方法将健康雄性C57/BL小鼠随机分为空白对照组、模型对照组、中药治疗组(YZXFD低、中、高质量组)和阳性对照组,采取腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)制备PD小鼠模型,应用免疫组织化学染色技术,观察各组小鼠黑质内nNOS、iNOS表达的变化情况。结果实验结果显示,在药物治疗后的第14、28天,中药治疗组小鼠与模型对照组小鼠比较,nNOS和iNOS基因表达都有不同程度的减少,尤其以YZXFD高剂量组效果明显,差异有统计学意义(P<0.01),分别减少38.6%和38.0%。结论YZXFD可通过抑制黑质多巴胺神经元内nNOS和iNOS的基因表达途径,减少神经元的氧化应激损伤,达到保护神经元的作用。

Objective To study the effects of the Yuzhenxifeng Decoction（YZXFD） on nNOS and iNOS expression in Parkinson disease（PD） mouse models. Methods Preparing PD model mice with MPTP. C57/BL mice were randomly divided into 4 groups：the blank control group, PD model group,Chinese medicine treatment group and positive control group. By using the immunohistochemical staining technique, we observed the change of expression of nNOS and iNOS in the SN of mice in all groups. Results The results showed that compared with model group, the expression of nNOS and iNOS gene in treatment group was lower than that in model group on 14th and 28th（P〈0.01）, and were 38.60% and 38.0% lower respectively. Conclusion YZXFD might exert its prevention and treatment effects on the PD through inhibiting nNOS and iNOS gene expression in the DA neurons.