摘要
本文报道了利用基因工程技术在大肠杆菌中表达人重组白细胞介素8(rh(?)。将质粒pGEM-hIL-8中的IL-8cDNA片段亚克隆到表达载体pMS31b中,在大肠杆菌中成功地高效表达出人IL-8融合蛋白,表达产物具有体内、体外对小鼠中性粒细胞的趋化活性。表明N端连入一段细菌蛋白并不影响IL-8的趋化活性。
By using genetic engineering techniques, we had successed in the expression of recombinant human interleukin 8 (rhIL-8)in E. col(?) IL-8 cDNA insert into the plasmid pGEM-IL-8 was subcloned into expression vector pMS316, and IL-8 fusion protein was produced in E.coli with high efficiency, The purified recombinant protein exhibited chemotactic activity for mouse neutrophils both in vive and in vitro. It showed that a fragment of the human IL-8 had no influence on the chemotactic activity of IL-8.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1991年第4期222-225,共4页
Chinese Journal of Immunology
基金
美国中华医学基金会资助课题
关键词
白细胞介素
人重组
基因工程
Recombinant human interleukin 8
Genetic engineering
Chemotactic activity
