蛹虫草多糖纯化工艺研究

Research on Technology of Purification Process of Cordyceps militaris Polysaccharide

为优化蛹虫草多糖的纯化工艺,以蛋白去除率和多糖保留率为指标,比较Sevage法、改良的Sevage法、澄清剂法三种方法对粗多糖中蛋白杂质的去除效果。本试验依次采用DEAE-52、Sephdex G-100葡聚糖凝胶柱层析进一步分离纯化蛹虫草多糖,再经Sephdex G-200柱色谱及高效液相凝胶色谱进行纯度检验。结果显示:澄清剂法蛋白去除率为93.4%,多糖保存率为90.7%;DEAE-52柱层析得到一个洗脱峰CP,再经Sephdex G-100柱层析后得到两个洗脱峰CP-1和CP-2。对CP-1和CP-2进行纯度检验,发现两者皆为均一性多糖。

To research the purification process of polysaccharide from Cordyceps militaris ,the effects of deproteinization with different methods（such as sevage ,improved sevage and present clarifier methods）were evaluated in terms of the ratio of protein removing and polysaccharide holding .Cordyceps militaris polysaccharide was separated and purified by DEAE‐52 and Sephdex G‐100 chromatography .The sample purity was checked by Sephdex G‐200 chromatography and HPLC . The results show that after purification by present clarifier methods ,the removal rate of protein was 93 .4% and the reten‐tion rate of polysaccharide was 90 .7% .The elution peak CP was obtained by DEAE-52 column chromatography and then the two elution peaks of CP -1 and CP -2 were obtained by Sephdex G -100 column chromatography .It was showed that the two elution peaks purified by column chromatography were both homogeneous polysaccharides through purity in‐spection .