木瓜蛋白酶对丝素蛋白的降解作用研究

Study on the Degradation of Papain on Silk Fibroin

丝素蛋白具有良好的生物相容性及降解性，被广泛地用于生物材料。本研究以废蚕茧为原料，通过脱胶、溶解、透析、浓缩等步骤制备了丝素蛋白溶液，再用木瓜蛋白酶降解丝素蛋白，利用SDS－聚丙烯酰胺凝胶电泳（SDS－PAGE）进行丝素蛋白多肽的鉴定，探讨pH、温度及酶活等因素对木瓜蛋白酶降解丝素蛋白影响。结果表明：在pH8～9、温度60～70℃，木瓜蛋白酶的活性为20～25 U时丝素蛋白水解效果较好，酶的作用时间越长效果越好。本研究为开发丝素蛋白在食品、化妆品中的提供了基础。

Silk fibroin is widely used in biomaterials based on its good biocompatibility and degradability .The silk fibroin solution is prepared by the steps of the process of degluing ,dissolution ,dialysis and concentration from waste cocoons . Then the fibroin is degraded by papain .The best condition of the degradation is explored by the means of SDS -PAGE for identifying the factors of pH ,temperature and papain activity .The results show that under the circumstances of pH being 8～9 ,temperature 60-70℃ ,and papain activity 20～25 U ,the hydrolysis effect of silk fibroin was the best ,and the longer time ,the better effect .