制药废水处理系统中耐盐微生物的种群动态及多样性分析

ANALYSIS OF POPULATION DYNAMICS AND DIVERSITY OF SALT-TOLERANT MICROORGANISMS IN A PHARMACEUTICAL WASTEWATER TREATMENT SYSTEM

采用A/O反应器模拟处理高盐度、高COD制药废水,并用PCR-TGGE技术对耐盐微生物驯化过程中微生物种群动态及多样性进行检测。结果表明:A/O反应器的可培养优势菌经16S r DNA序列分析,分别为Klebsiella(克雷伯氏杆菌属)、Morganella(摩根氏菌属)、Aeromonas(气单胞菌属)和Rhodococcus(红球菌属);反应器中微生物种群丰富,可通过驯化得到耐高盐高COD的优势菌群;缺氧池的菌群抗盐度冲击负荷不如好氧池强,不同盐度下缺氧池菌群相似性比好氧池更高,好氧菌和兼氧菌在高盐废水处理中较厌氧菌具有更高的优势地位。

A / O reactor was used to treat pharmaceutical wastewater with high salt and COD,and the population dynamics and diversity of salt-tolerant microorganisms was determined by PCR-TGGE. The results showed that： According to 16 S r DNA sequence analysis,A / O＇ s culturable dominant bacteria were Klebsiella,Morganella,Aeromonas and Rhodococcus; microbial populations in the reactor had a high diversity,and the dominant bacteria had the characteristics of the high salt and high COD by culturing; the anarobic pool was lower than aeration pool in salinity impact load,the bacteria similarity in anarobic pool was higher than aeration pool under different salinity conditions,aerobic and facultative bacteria had a more dominant position than anaerobic bacteria in the high-salt wastewater treatment system.