ARTP诱变选育鼠李糖脂高产菌及鼠李糖脂促进枯草芽孢杆菌产纤维素酶的研究

Screening of High-yield Rhamnolipid Producing Strain by ARTP and the Effect of Rhamnolipid on the Cellulase and Xylanase

旨在选育鼠李糖脂高产菌株,以实验室筛选的产鼠李糖脂的Pseudomonas aeruginosa C3为出发菌株进行常压室温等离子体诱变(ARTP),选育出一株高产突变株Pseudomonas aeruginosa SC-11,产量比出发菌株提高了74.1%。进一步对产鼠李糖脂的摇瓶发酵培养基和发酵条件进行了优化,优化后的鼠李糖脂产量达42 g/L,底物转化率达到0.7 g/g底物。添加0.01%鼠李糖脂到Bacillus subtilis CL产羧甲基纤维素酶与木聚糖酶的培养基中,羧甲基纤维素酶活与木聚糖酶活分别提高12.9%和18.3%。研究表明,鼠李糖脂通过增加细胞通透性来提高胞外酶产量。

In order to obtain an industrial strain with higher rhamnolipid production, the original strain of Pseudomonas aeruginosa C3 was mutated by atmospheric and room temperature plasmas. A good genetic stability strain SC-11 was screened from the mutants, which produced rhamnolipid 74.1% higher than that by the original strain. Through single factor experiments, the culture medium for rhamnolipid production was opttimized. The yield of rhamnolipid could reach 42 g/L and the substrate conversion rate is 0.7 g/g substrates. 0.01% rhamnolipid could enhance CMCase by 12.9% and xylanase by 18.3%. The results show that rhamnolipid increased the production of cellulase by increasing cell permeability.