大菱鲆病原鳗弧菌的检验与分析

Examination and Analysis on Pathogenic Vibrio anguillarum from Turbot Scophthalmus maximus

旨对从发病死亡的养殖大菱鲆鱼苗中分离的10株细菌进行检验与分析。运用表观分类学指征、16S r RNA基因序列与系统发育、免疫血清学、人工感染的致病作用等方法进行检验。经过检验,结果表明为弧菌属(Vibrio Pacini 1854)的鳗弧菌(Vibrio anguillarum Bergeman 1909),对供试大菱鲆显示强致病性;与用不同来源的鳗弧菌制备的全菌体抗血清能够发生免疫血清学的强凝集反应。

Ten strains isolated from died larve of cultured turbot were examined and analyzed. 10 strains were detected by phenotypic information, detection and phylogenetic analysis of the 16 S r RNA gene sequence, serological, as well as pathogenic role of artificial model infection. It was found that Vibrio anguillarum showed strong pathogenicity on turbot, which was tested with various sources Vibrio anguillarumantiserum and occurred strong serological agglutination on immunity.