酿酒葡萄品种耐盐性的研究

Evaluation of Salt Tolerance of Wine Grapes

以引进的11个酿酒葡萄品种为试材,研究了不同葡萄品种在不同浓度NaCl条件下的盐害指数、株高和根数等指标,从中筛选出4个耐0.4%NaCl的品种,以其中耐盐性较强、中等和较弱的3个品种进一步分析了其在不同盐胁迫下脯氨酸和丙二醛(malondialdehyde,MDA)含量及超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase dismutase,POD)活性的变化。结果表明:在盐胁迫下,3个葡萄品种叶片MDA含量均有不同程度升高,耐盐性较弱的品种‘Dechaunac’叶片中MDA含量增加幅度大于抗盐性较强的‘Vidal Blanc’和‘Villard Blanc’品种;在0.2%NaCl条件下,3个品种SOD和POD活性都有不同程度提高,当盐浓度为0.4%时,耐盐性较弱的品种‘Dechaunac’叶片中SOD、POD活性开始下降,而‘Villard Blanc’和‘Vidal Blanc’品种SOD、POD活性继续上升,表明维持较强的抗氧化能力是其抵御盐分胁迫的重要方式。盐胁迫下3个品种脯氨酸含量均有不同程度升高,但与抗盐性无严格相关性。

Taking eleven imported wine grapes as materials,the salt tolerance of different grapes was tested,four varieties with relatively stronger salt tolerance were screened according to salt injury index,shoot high as well as root numbers under different salt concentration.The proline and malondialdehyde（MDA）contents as well as superoxide dismutase（SOD）and peroxidase dismutase（POD）activities of three grapes with different salt tolerance were examined.The results showed that the MDA content of three varieties all increased,but that of‘Dechaunac＇with weak salt tolerance increased more significantly.SOD and POD activity of‘Vidal Blanc＇and‘Villard Blanc＇with high salt tolerance keep increasing under different salt concentration,that of‘Dechaunac＇increased under 0.20% NaCl whereas decreased under 0.40%NaCl,this result indicated that ‘Villard Blanc＇and‘Vidal Blanc＇keeping high antioxidant enzyme activity was an important way for wine grape to resist oxidation induced by salt stress.The proline content of three varieties increased at different level under salt stress,but there was not positive correlation between proline content and salt tolerance.