一株石油烃降解菌的固定化及其降解特性研究

Immobilization and Degradation Characteristics of A Petroleum-Degrading Strain

从石油污染盐渍土壤中筛选出一株对液蜡乳化效果明显、对原油降解率达56.8%的菌株,命名为BZ-L。经生理生化和16SrRNA序列分析,初步鉴定该菌株属于沙雷氏菌属。以海藻酸钠和活性炭为包埋剂,对该菌株进行固定化研究。结果表明,当活性炭含量为0.8%时,固定化微球的破损率最低、渗透性最好;在接种量为35.0g·L-1、NaCl浓度为6.0%时,固定化微球对原油的降解率可达61.7%;菌株BZ-L的固定化微球对原油的降解率明显高于游离菌,且比游离菌的耐盐性能更强,可用于石油污染盐渍土壤的生物修复。

A petroleum-degrading strain named as BZ-L was isolated from the petroleum contaminated saline soil,which could emulsify liquid paraffin well and degradation rate of petroleum was 56.8%.The strain was belonged to Serratia marcescens,according to physical and biochemical characteristics and 16 S rRNA sequence analysis.Sodium alginate and activated carbon were employed as embedding agents to study the immobilization of the strain.Results showed that damage rate of the immobilized microspheres were the lowest and the permeability was the best when content of activated carbon was 0.8%.The degradation rate of petroleum by the immobilized microspheres was 61.7%,with the inoculation amount of 35.0g·L-1 and the NaCl concentration of6.0%.The degradation rate of petroleum by the immobilized microspheres was higher than that of the free strain,and they were more halotolerant.Strain BZ-L and the immobilized microspheres could be used in the bioremediation of the petroleum contaminated saline soil.