酶解桃仁分离蛋白制备肾素和ACE双重抑制肽的工艺研究

Study on enzymatic hydrolysis conditions for the preparation of dual rennin and ACE inhibitory peptide from peach kernel protein isolate

以桃仁分离蛋白(PKPI)为原料,采用碱性蛋白酶水解桃仁分离蛋白制备了肾素和ACE双重抑制肽.以体外ACE抑制率和肾素抑制率为指标,通过单因素实验对酶解温度、酶解pH值、底物质量浓度和加酶量等酶解工艺参数进行了研究,并利用响应面法优化了酶解工艺.结果表明,碱性蛋白酶水解桃仁分离蛋白制备肾素和ACE双重抑制肽的最佳工艺参数为:酶解温度50.6℃、酶解pH值8.02、加酶量3.06%,所得到的酶解产物ACE抑制率为69.03%、肾素抑制率为57.61%.

The preparation of dual rennin and angiotensin‐I‐converting enzyme （ACE） inhibi‐tory peptides from peach kernel protein isolate with Alcalase 2 .4 L was investigated .ACE in‐hibitory rate in vitro and renin inhibitory rate in vitro were investigated with respect to hy‐drolysis parameters including temperature ,pH ,substrate concentration and enzyme dosage . As a result ,the optimum conditions for enzymatic preparation of dual rennin and ACE inhib‐itory peptides from peach kernel protein isolate were found to be hydrolysis at pH 8 .02 ,tem‐perature of 50 .6 ℃ and enzyme dosage of 3 .06% .Under these conditions ,the prepared pep‐tides showed an ACE inhibitory rate of 69 .03% ,renin inhibitory rate of 57 .61% .