芹菜素对人肝癌细胞Hepg2增殖与葡萄糖摄取的影响

Effects of Apigenin on Proliferationof Human Hepatocellular Carcinoma Hepg2 Cells and Glucose Uptake

目的:探讨芹菜素对人肝癌Hepg2细胞增殖与葡萄糖摄取的影响。方法:以Hepg2细胞为研究对象,实验均用0.625~10μmol·L^(-1)不同浓度芹菜素作用于细胞,细胞密度以6×10~3个/m L接种于96孔培养板,5复孔/组,分别培养24,48,72 h后,MTT法检测细胞增殖抑制情况;同时,取96孔板培养24,48,72 h的细胞上清液,用葡萄糖测定试剂盒检测Hepg2细胞葡萄糖摄取情况;细胞密度以10~6个/m L接种于6孔培养板中,培养48 h后,Western blot检测葡萄糖转运蛋白1(GLUT1)表达的变化。结果:与对照组比较,芹菜素各浓度组均能抑制Hepg2细胞的增殖,且抑制作用呈时间和浓度依赖性,差异有统计学意义(P<0.05,P<0.01);芹菜素各浓度组作用后Hepg2细胞对葡萄糖的摄取明显减少(P<0.05,P<0.01);与对照组比较,GLUT1蛋白随着芹菜素浓度的升高表达减少(P<0.05,P<0.01)。结论:芹菜素具有抑制人肝癌Hepg2细胞增殖与葡萄糖摄取的作用,为肝癌的预防和治疗提供新的思路。

Objective： To investigate the effect of apigenin on proliferationof human hepatocellular carcinoma Hepg2 cells and glucose uptake. Method： Human hepatocellular carcinoma Hepg2 cells were used as study objects and apigeninof different concentrations（ 0. 625-10 μmol·L^-1） acted on the cells. The cells were inoculated at 6 × 10^3/ m L in 96-well plate,5 duplicate wells / group. After culture for 24,48,72 h,cell proliferation was detected by MTT. Glucose uptake was detected by glucose test kit. The cells were inoculated at10^6/ m L in 6-well plate,and after culture for 48 h,the changes in glucose transporter protein 1（ GLUT1） was detected by Western blot. Result： The proliferation of human hepatocellular carcinoma Hepg2 cells could be inhibited by apigenin compared with the control group,and the inhibitory effect was in a time and concentration dependent manner, with statistically significant difference（ P〈 0. 05, P 〈0. 01）. Apigeninof different concentrations could significantly reduce the intake of glucose by Hepg2 cells（ P〈 0. 05,P 〈0. 01）. Compared with the control group,GLUT1 expression was decreased with the increase of apigenin concentration（ P 〈0. 05,P 〈0. 01）. Conclusion： Apigenin of different concentrations could inhibit the proliferation of human hepatocellular carcinoma Hepg2 cells and glucose uptake.