通莲汤对裸小鼠胃癌移植瘤组织形态学及外周血免疫因子的影响

Effect of Tonglian Decoction on Histomorphology of Transplanted Tumor and Immune Factors in Peripheral Blood of Nude Mice

目的:研究通莲汤对胃癌MGC803细胞荷瘤裸鼠组织形态学及对外周血免疫因子肿瘤坏死因子-α(TNF-α),白细胞介素6(IL-6)和IL-8的影响作用。方法:胃腺癌MGC803细胞接种于Balb/C裸小鼠右前肢皮下,建立荷瘤模型,随机分为模型组(生理盐水10 m L·kg-1),消癌平组(1.8 g·kg-1),通莲汤高、中、低剂量组(1.5,0.75,0.37 g·kg-1),各组连续给药3周,再连续观察3周,6周后处死小鼠,分离瘤组织,HE染色,光镜观察组织形态结构;裸鼠眼球取血,分离血清,ELISA法检测血清中TNF-α,IL-6和IL-8含量。结果:高剂量通莲汤干预的裸鼠移植瘤瘤体微结构淡染,核分裂少见,中心出现大片坏死区;中、低剂量通莲汤干预的细胞组织形态学变化甚微。高剂量通莲汤可显著下调裸鼠外周血清TNF-α,IL-6和IL-8水平,分别为(33.82±14.43)ng·L-1,(45.49±15.61)ng·L-1和(132.09±29.51)ng·L-1,与模型组比较有显著性差异(P<0.05,P<0.01)。结论:较高剂量的通莲汤可促进裸鼠胃癌移植瘤组织坏死,其抑瘤机制与下调血清TNF-α,IL-6和IL-8等因子水平,增强免疫调节有关。

Objective： To observe the effect of Tonglian decoction（ TD） on histomorphology of transplanted tumor and tumor necrosis factor-α（ TNF-α）,interleukin（ IL）-6,and IL-8 in peripheral blood of nude mice inoculated with gastric cancer MGC803 cells. Method： All mice were inoculated subcutaneously with MGC803 cells in the right forelimbs and were randomly divided into the following groups,the model group（ saline solution,10 m L·kg^- 1）,the Xiaoaiping group（ 1. 8 g·kg^- 1） and high-,middle-,low-dose TD groups（ 1. 5,0. 75,0. 37 g·kg^- 1）. After 3-week administration,they were observed for another 3 weeks continuously. The hematoxylin and eosin（ HE） stain of the tumor tissue was made followed the observation on micro-structure by fluorescence microscope. The concentrations of serum TNF-α,IL-6 and IL-8 were examined by enzyme linked immunosorbent assay（ ELISA） method. Result： In the high-dose TD group,HE pathological changes showed large necrotic area in slice center,accompanied with stained tumor cells in light color and few cellular nuclear division.The high-dose TD could down-regulate the contents of TNF-α,IL-6 and IL-8 in peripheral serum of nude mice with the values of（ 33. 82 ± 14. 43）,（ 45. 49 ± 15. 61）,（ 132. 09 ± 29. 51） ng·L^- 1,respectively,showing statistical difference when compared with the model group（ P 〈0. 05,P 〈0. 01）. Conclusion： High-dose TD could promote the tumor necrosis of mice inoculated with MGC803 cells. The mechanism may be achieved by down-regulating the contents of TNF-α,IL-6 and IL-8 in peripheral serum and strengthening the immune ability.