痫愈痊颗粒对戊四氮致痫大鼠海马Glu,GABA含量的影响

Effects of Xianyuquan Granules on Contents of Aminobutyric Acid and Glutamic Acid in Hippocampus of Rats with Epilepsy Caused by Pentetrazol

目的:观察痫愈痊颗粒对戊四氮致痫大鼠海马谷氨酸(Glu),γ-氨基丁酸(GABA)含量的影响,探讨痫愈痊颗粒治疗癫痫的机制。方法:将70只健康雄性SD大鼠随机分为正常组、模型组、丙戊酸钠组(200 mg·kg-1)、痫愈痊颗粒低、高剂量组(586,1 172 mg·kg-1),采用亚惊厥剂量戊四氮(PTZ)ip建立癫痫大鼠模型,在造模同时即开始给药,共28 d。行为学观察后取海马组织,采用高效液相色谱-四级杆离子阱串联质谱仪检测海马中Glu,GABA的含量。结果:59只大鼠进入结果分析,与模型组比较,痫愈痊颗粒与丙戊酸钠干预后发作潜伏期延长、惊厥持续时间缩短(P<0.01);惊厥持续时间高剂量组优于低剂量组(P<0.05)。模型组与正常组相比,海马Glu含量明显上升,GABA含量明显下降(P<0.01);与模型组比较,痫愈痊颗粒高、低剂量组和丙戊酸钠组可明显降低海马Glu含量,升高GABA含量(P<0.05,P<0.01)。结论:采用亚惊厥剂量PTZ能够建立癫痫大鼠模型;痫愈痊颗粒抗癫痫机制可能与其降低海马Glu含量,增加GABA含量有关。

Objective： To observe the effect of Xianyuquan granules（ JYQG） on the contents of aminobutyric acid（ GABA） and glutamic acid（ Glu） in hippocampus of rats with epilepsy caused by pentetrazol,and explore the mechanism of JYQG in treatment of epilepsy. Method： Seventy healthy male SD rats were randomized into normal group,model group,valproic acid（ VPA） group（ 200 mg·kg^- 1）,low-dose and high-dose JYQG groups（ 586,1 172 mg·kg^- 1）. Subconvulsant dose of pentetrazol（ PTZ） was ip administered to establish epileptic rat models. The others were administrated with their medicine respectively for twenty-eight days.Hippocampus tissues were taken after behavior observation. The contents of GABA and Glu in hippocampus of the rats were measured on HPLC-MS / MSQ-TRAP. Result： Fifty-nine rats were analyzed finally. Compared with the model group,the incubation period of the VPA treated group and JYQG treated group was prolonged and the convulsion duration was shorter（ P 〈0. 01）,convulsion duration in high dose group was shorter than that in low dose group（ P 〈0. 05）. Glu content in the hippocampus of model group was significantly higher than that of the normal group and the GABA content was significantly less than that of the normal group（ P〈 0. 01）. Glu content in rats administrated with corresponding medicines was significantly less than that of model group while GABA content was higher than that of model group（ P 〈0. 05, P〈 0. 01）. Conclusion： The application of subconvulsant-dose pentetrazol can establish the epileptic model. The anti-epileptic effect of JYQG may be associated with decreasing the content of Glu and increasing the content of GABA in the hippocampus.