石榴果实乙酸乙酯提取物对胰岛素抵抗大鼠血糖及血脂的干预作用

Intervention Effects of Ethyl Acetate Extract of Punica granatum Fruit on Blood Glucose and Blood Lipid in Rats with Insulin Resistance

目的:观察石榴果实乙酸乙酯提取物(PGFEAE)对正常大鼠及胰岛素抵抗(IR)大鼠模型血糖、血脂的干预作用,评价其改善胰岛素抵抗的药理作用。方法:高糖高脂饲料喂养加链脲佐菌素(STZ)2次ip(30 mg·kg-1)建立胰岛素抵抗大鼠模型。分别以PGFEAE 100,200,300 mg·kg-1ig干预4周后,检测空腹血糖(FBG)、空腹胰岛素水平(FINS)、胰岛素敏感指数(ISI)、血清游离脂肪酸(FFA)浓度、甘油三酯(TG)浓度。结果:与正常组相比,模型组FBG,FINS,FFA,TG水平显著上升,胰岛素(INS)水平下降(P<0.05,P<0.01);造模4周后,正常组与模型组大鼠FBG,FINS,TG分别为5.94,30.71,0.57mmol·L-1;13.05,42.36,1.62 mmol·L-1;给药4周后,与模型组相比,PGFEAE各剂量组均可有效降低胰岛素抵抗大鼠模型FBG,FINS,FFA,TG水平,使胰岛素抵抗大鼠模型INS水平升高(P<0.05,P<0.01)。结论:PGFEAE可有效改善胰岛素抵抗大鼠血糖、血脂偏高的症状,其对正常大鼠血糖、血脂无干预作用。

Objective： To observe the intervention effect of ethyl acetate extract of Punica granatum fruit（ PGFEAE） on blood glucose and blood lipid in rats with insulin resistance（ IR） and normal rats,and evaluate its pharmacological effects on improving insulin resistance. Method： High-sugar and high-fat diet plus intraperitoneal injection of streptozocin（ STZ） twice（ 30 mg·kg^- 1） to establish rat models of insulin resistance.After 4 weeks of ig administering 100,200,300 mg·kg^- 1 PGFEAE respectively,fasting blood glucose（ FBG）,fasting insulin（ FINS）, insulin sensitivity index（ ISI）, serum free fatty acid（ FFA） concentration, and triglyceride（ TG） concentration were measured. Result： Compared with the normal group,FBG,FINS,FFA,and TG levels were significantly higher and ISI level was lower in model group（ P 〈0. 05,P〈 0. 01）,after 4 weeks of modeling,FBG,FINS,and TG levels were 5. 94,30. 71,0. 57 mmol·L^- 1. 13. 05,42. 36,1. 62 mmol·L^- 1 respectively in normal group and model group. After 4 weeks of treatment,various dose groups of PGFEAE can effectively reduce FBG,FINS,FFA,and TG levels and increase ISI level in rats with insulin resistance compared with the model group（ P〈 0. 05,P 〈0. 01）. Conclusion： PGFEAE can effectively improve the symptoms of high blood glucose and blood lipid in rats with insulin resistance.