三七多糖对大鼠脑缺血再灌注损伤的保护作用

Notoginseng polysaccharide on rat ischemia-reperfusion injury of protection

目的探讨三七多糖对大鼠脑缺血再灌注损伤的保护作用及可能机制。方法雄性Wistar大鼠随机分为5组,假手术组、模型组、尼莫地平组(10 mg/kg)和三七多糖高[300 mg/(kg·d)]、低[100 mg/(kg·d)]剂量组。采用线栓法栓塞大鼠大脑中动脉建立大鼠脑缺血再灌注损伤模型,术前15 d灌胃给药,每天1次。再灌注结束后测定脑组织含水量、脑梗死面积比,脑组织MDA、GSH-Px、SOD、TNF-α、IL-1β和IL-10水平。结果与模型组比较,三七多糖可以减少脑组织含水量及脑组织梗死面积比(P<0.05 or P<0.01);同时,与模型组比较,三七多糖能使脑缺血再灌注损伤大鼠脑组织的GSH-Px、SOD活性和IL-10含量升高,降低MDA和TNF-α、IL-1β含量(P<0.01)。结论三七多糖对大鼠脑缺血再灌注损伤具有一定的保护作用,该保护作用与其提高脑组织抗氧化能力、抑制炎症因子的过度产生有关。

Objective Explore the notoginseng polysaccharides on the protective effects of ischemia reperfusion injury in rats and the possible mechanism. Methods The male Wistar rats were randomly divided into 5 groups,control group,model group,nim horizon（ 10 mg / kg） and notoginseng polysaccharides high [300 mg /（ kg·d） ],low dose [100 mg /（ kg·d） ]group. By using line switch embolism in the rat middle cerebral artery in ischemia-reperfusion injury rats model was established,preoperative 15 d lavage,1 times a day. Reperfusion after the determination of brain tissue water content,cerebral infarction area ratio,brain tissue MDA,gsh-px,SOD,TNF alpha,beta IL-1 and IL-10 levels. Results Compared with model group,notoginseng polysaccharides can reduce the brain water content and cerebral infarction area ratio（ P〈0. 05 or P〈0. 01）; At the same time,compared with model group,notoginseng polysaccharides can make the cerebral ischemia reperfusion injury in rats had gsh-px,SOD activity and IL-10 content increases,reduce the MDA and TNF alpha and beta IL-1 content（ P〈0. 01）. Conclusion Notoginseng polysaccharides on ischemia-reperfusion injury rats has a certain protective effect,the protective effect and its improving brain tissue antioxidant capacity,inhibit the excessive production of inflammatory cytokines.