雌激素受体基因甲基化与脑出血的关系

Relationship between gene methylation of estrogen receptor gene and cerebral hemorrhage

目的:探讨伴或不伴颈动脉粥样硬化脑出血与雌激素受体α(ER-α)基因启动子区甲基化状态的关系,方法:入选124例脑出血(经颈动脉超声检查证实部分有颈动脉粥样硬化)的患者为实验组,47例健康体检者为对照组,采集上述入选病例和对照组肘静脉血;提取DNA,采用巢式甲基化特异性PCR(n MSP)检测ER-α基因启动子区甲基化状态。结果:脑出血组甲基化发生率显著高于对照组,差异具有统计学意义(X2=14.090,P=0.000)。颈动脉有无粥样硬化的脑出血患者甲基化率差异无统计学意义。结论:脑出血患者ER-α基因启动子区甲基化可能与动脉粥样硬化有关,是促进脑出血发病的机制之一,颅内动脉粥样硬化与颈动脉硬化可能不平行。

Objective ： To study the relationship between the methylation status of the promoter region of the estrogen receptor α （ER-α） gene in the patients and cerebral hemorrhage associated with carotid atherosclerosis or not, Methods ：124 patients with cerebral hemorrhage （They are partly carotid atherosclerosis by color Doppler ultrasound examination） and 47 healthy persons were selected for ER-ctgene methylation research. Their blood of the elbow was collected for DNA extraction, The ER-ctgene promoter methylation status were detected by nested methylation specific PCR （nMSP）.Results：The methylation of cerebral hemorrhage group was significantly higher than that in normal control group,The difference was statistically significant （X=14.090,P=0.000）.There was no statistical significance in the methylation rate of patients with carotid atherosclerosis or not. Conclusions：The methylation of ER-α gene promoter region in patients may be related to the atherosclerosis,which may suggest an involvement of cerebral hemorrhage . It may not be the same in intracranial atherosclerosis and carotid atherosclerosis.