食蟹猴子宫内膜异位症动物模型的建立

Establishment of an animal model of endometriosis in macaca fascicularis

目的构建食蟹猴子宫内膜异位症动物模型。方法将15只雌性食蟹猴分为两组,实验组(n=10)行手术获取子宫内膜组织,种植到自体盆腔大网膜、子宫直肠窝、子宫表面等处;对照组(n=5)予以大网膜组织代替子宫内膜组织种植。分别于手术后第8、16周行腹腔镜探查,取可疑病灶行病理学检查。结果实验组建模成功8只食蟹猴,可疑病灶行组织病理检查可见完整腺体和间质细胞结构;5只对照组食蟹猴建模失败。结论成功构建了非人灵长类动物食蟹猴子宫内膜异位症模型,为子宫内膜异位症的研究增加了新的动物选择。

Objective To establish an animal model of endometriosis in macaca fascicularis. Methods Fifteen female macaca fascicularises were selected. Surgery was performed in the experimental group to collect endometrial tissues, which were then implanted into the pelvic omentum majus, douglas pouch, as well as uterus surface. A similar surgery was operated on the control group, in which the omentum majus was collected and implanted instead. Laparoscopic exploration was performed eight weeks and sixteen weeks postoperatively, followed by histopathological examination of suspected lesions. Results In the experimental group, eight female macaca fascicularis models of endometriosis were successfully established. Histopathological examination of suspected lesions revealed complete structure of the glands and stromal cells. Model establishment failed in the 5 subjects of the control group. Conclusion The endometriosis model of macaca fascicularis was successfully established, which adds a new animal choice to the research of endometriosis.