血浆WIF-1 DNA甲基化与肝癌患者临床特征和预后的关系

Expression and clinical significance of WIF-1 DNA methylation in hepatic cancer

目的:检测肝癌患者血浆WIF-1甲基化状态,研究其与临床各相关因素的关系及对肝癌患者预后的意义。方法:采用甲基化特异性PCR法检测肝癌患者和健康体检者血浆中提取DNA的WIF-1甲基化状态,结合临床特征(包括性别、年龄、肝硬化、AFP水平、Hbs Ag及临床分期)进行分析,探讨联合甲基化状态与临床特征之间的关系,分析其与肝癌患者复发转移和预后的关系。结果:96例肝癌患者血浆WIF-1甲基化阳性为31.4%,对照健康组无甲基化,差异有显著的统计学差异(P<0.05)。肝癌血浆WIF-1甲基化状况与病人性别、年龄、抗-HBs、肝硬化无相关性(P>0.05),与AFP水平、肿瘤大小、淋巴结转移及TNM分期相关(P<0.05)。WIF-1甲基化阳性组较阴性组转移复发率高,存活率较甲基化阴性组低。结论:WIF-1基因甲基化是影响肝癌患者FPS(progression-free survival)和OS(overall survival)的危险因素,对肝癌复发转移预测具有重要的价值,有望成为评估肝癌预后的有效指标。

Objectives： Our topic is to analysis the relationship between the promoter region WIF-1 DNA methylation status and the characteristics in hepatic cancer, and explores the role ofWIF-1 DNA methylation in the occurrence and metastasis of hepatic cancer. Methods： TkLe methylation specific-PCR （MSP） was used to detect the tumor suppressor gene W-IF-1 methylation in the above-mentioned tissues. To explore the role of them in the occurrence and development of hepatic cancer, and the relationship associated with the clinical relevant factors. Discussion the relationship between the methylation status with clinicopathologic factors （sex, age, AFP, differentiation degree, clinical stage） and progression-free survival （PFS） as well as overall survival （OS） were analyzed. Results： The methylation status of W-IF-1 in plasma of hepatic cancer patients were 31.4%. There were no correlations among the WIF-1 methylation status in hepatic cancer plasma and patient＇s sex, age, cirrhosis and anti-HBs （P〉0.05）, but correlate with degree of differentiation TNM staging. Multivariate analysis indicated that besides tumor size,methylation status is also an independent risk factor influencing recurrence, also independently correlated with TrP and OS in hepatic patients. Conclusion＂ WIF-1 methylation is independently correlated with TrP and OS in hepatic cancer patients, and combined detection of methylation of gene show great importance in the field of judging the prognosis. Tumor suppressor gene WIF-1 methylation is an early molecular event of hepatic cancer.