饮用淡化海水对大鼠肝脏合成及代谢功能的影响

Effect of Drinking Desalinated Seawater on Liver Synthetic Function and Bilirubin Metabolism in Rats

目的观察饮用淡化海水对大鼠胆红素代谢、胆汁淤积指标及肝脏合成功能的变化情况,推测饮用淡化海水是否会对大鼠肝功能产生影响。方法通过对饮用淡化海水的大鼠进行为期1年的实验,选取临床常用、重要的胆红素代谢及胆汁淤积指标总胆红素(TBIL)、碱性磷酸酶(ALP)及肝脏合成功能指标血清白蛋白(ALB)、胆碱酯酶(CHE),分别在饮用90、180和365 d 3个不同时间点采集血液样品,通过全自动生化分析仪对上述指标进行测定分析。结果受试大鼠在实验起始(0 d)时各组ALP活性均处于高水平状态,90 d时,血中ALP活性水平均出现急剧下降,此后随着时间延长各组大鼠血中ALP变化不明显。饮用淡化海水90和365 d的雌性大鼠血中ALP活性水平明显低于对照组(P<0.05)。各组大鼠血中TBIL含量与对照组相比无明显差异。在受试365 d时,饮用淡化海水组雌、雄性大鼠血中ALB含量及CHE活性均明显低于对照组(P<0.05),而在其他各时间点中仅雄性大鼠饮用淡化海水180 d时,其血中ALB含量低于对照组,有统计学差异(P<0.05)。结论饮用淡化海水组雌性大鼠血中ALP变化可能与饮用淡化海水组雌性大鼠体内雌激素的变化具有相关性,且与骨骼代谢机制相关联。可以认为长期饮用淡化海水可能会引起大鼠血中CHE活性的降低而对大鼠体内TBIL无明显影响。

Objective To observe the effect of drinking desalinated seawater on liver synthetic function and bilirubin metabolism in rats. Methods Important liver function indexes,such as serum albumin（ ALB）,cholinesterase（ CHE）,alkaline phosphatase（ ALP） and total bilirubin（ TBIL） of Wistar rats drinking tap water（ control group） or desalinated seawater（ test group） for 0,90,180 and 365 d,were analyzed by automatic biochemical analyzer. Results The activity of ALP in rats drinking desalinated seawater at the start（ 0 d） was high,and it was dropped sharply at the 90 thday of the experiment（ P〈0. 05）,and no obvious change of ALP was observed in the later time. No obvious difference of TBIL was found between the control group and the test group. Serum ALB and the activity of CHE of female rats drinking desalinated seawater for one year was lower than the control group（ P〈0. 05）.Serum ALB of male rats drinking desalinated seawater for 180 d was lower than the control group（ P〈0. 05）. Conclusions The influence of drinking desalinated seawater on the activity of ALP in female rats might be related to the change of estrogen,which might be associated with the mechanism of bone metabolism. It could be considered that the activity of serum CHE of rats drinking desalinated seawater for a long time might be reduced,and no influence on TBIL of rats.