氚-标记胸腺嘧啶核苷掺入法测定转化生长因子β_1对视网膜色素上皮细胞增殖的影响

Effect of transforming growth factor β_1 on the proliferation of human retinal pigment epithelium by the detection of ~3H-thymidine deoxyribose absorption

目的:用氚-标记胸腺嘧啶核苷(~3H-TdR)掺入法观察转化生长因子β_1(TGF-β_1)对人视网膜色素上皮细胞(retinal pigment epithelium cells,RPE)增殖的影响,探讨TGF-β_1和RPE细胞在增殖性玻璃体视网膜病变发病机制中的作用。方法:RPE细胞体外培养,用不同浓度的TGF-β_10.1、1.0、5.0、10.0和100.0μg/L对细胞进行处理,~3H-TdR掺入法测定细胞放射性强度。结果:TGF-β_10.1、1.0、5.0、10.0μg/L作用后RPE活细胞数增加,细胞~3H-TdR摄入量均较对照组显著增加,TGF-β_1100.0μg/L对RPE作用相反,细胞~3H-TdR摄入量明显低于对照组(P<0.01)。结论:TGF-β_1对人RPE细胞增殖有双相调节性,其不同作用的发挥依赖TGF-β_1的浓度,其中TGF-β_1促RPE细胞增殖作用是诱导增殖性玻璃体视网膜病变发生的可能机制之一。

Objective： To investigate the effects of transforming growth factorβ1（ TGF-β1） on the proliferation of human retinal pigment epithelium（ RPE） cells by3H- thymidine deoxyribose（3H-TdR） absorption,and explore the pathogenesis mechanism of vitreoretinopathy. Methods： Human retinal pigment epithelium cells were cultured in vitro,the cells were incubated with 0. 1,1. 0,5. 0,10. 0 and 100. 0 μg / L of TGF-β1. The radioactivity intensity of cells were determined by^3H-TdR absorption. Results： The 0. 1,1. 0,5.0 and 10. 0 μg / L of TGF-β1could promote the proliferation of RPE cells to increase the number of living cells,the intaking3H-TdR value of cells was significantly higher than that in control group（ P〈0. 01）. The effects of the 100. 0 μg / L of TGF-β1on the proliferation of RPE cells was opposite,the intaking^3H-TdR value of cells was significantly lower than that in control group（ P〈0. 01）.Conclusions： The effect of TGF-β1on the proliferation of RPE cells is dual regulation,different concentrations of TGF-β1may have different effects on RPE cells. The effect of TGF-β1on promoting the proliferation of RPE cells maybe one of mechanisms of proliferative vitreoretinopathy.