宝安区不同年龄健康成人血浆D-二聚体生物参考区间的建立

Establishment of biological reference intervals of plasma D-dimer for healthy adults of different ages in Bao′an District

目的建立宝安区不同年龄健康成人血浆D-二聚体生物参考区间,为临床诊断和治疗提供更科学、更有价值的参考依据。方法选取2014年6月至2015年1月深圳市宝安区松岗人民医院体检科不同年龄健康成人共1 156例,其中男417例,女739例。按年龄分为18-50岁、〉50-60岁、〉60岁3组。采用Sysmex CA1500全自动凝血分析仪免疫比浊法检测血浆D-二聚体水平,采用SPSS19.0软件对数据进行统计分析,血浆D-二聚体水平呈偏态分布,采用百分位数（P95）表示参考区间的单侧上限,确定相应的生物参考区间。并分析比较不同年龄和不同性别之间血浆D-二聚体水平之间的差异。结果同年龄组男性和女性D-二聚体水平比较差异无统计学意义（t=0.372-1.092,P〉0.05）;18-50岁和大于50-60岁组比较,血浆D-二聚体水平之间差异无统计学意义（t=0.954,P〉0.05）,〉60岁组与其他两组比较,血浆D-二聚体水平之间差异有统计学意义（t=5.972,P〈0.05）;18-60岁D-二聚体正常参考区间为0-225mg/L;〉60岁D-二聚体正常参考区间为0-297mg/L。结论血浆D-二聚体水平与性别无关,与年龄有关,随年龄增高D-二聚体水平呈上升趋势。不同人群、不同仪器使用配套试剂应建立自己的正常值参考区间。

Objective To establish the biological reference intervals of plasma D-dimer for healthy adults of different ages in Bao′an District,so as to provide more scientific and valuable reference for diagnosis and treatment in clinic.Methods 1 156 healthy adults of different ages were selected in Department of Medical Examination,Songgang People′s Hospital of Bao′an District,from June 2014 to January 2015,there were 417 males and 739 females among which.According to their ages,these healthy adults were divided into three groups：18-50 years old group,〉50-60 years old group and 〉60 years old group.Plasma D-dimer was detected by immune turbidimetric method on Sysmex CA1500 automatic blood coagulation analyzer.SPSS19.0software was used for the statistic analysis of the results.The unilateral upper limit of D-dimer reference interval was represented by percentile（P95）,since the detection results of D-dimer was skewed distribution.The appropriate biological reference intervals were determined,and the differences of D-dimer levels among adults of different ages and different genders were analyzed and compared.Results In the same age group,there was no significantly difference of the D-dimer level between males and females（t=0.372-1.092,P〉0.05）.There was no significant difference of the D-dimer level between 18-50 years old group and 〉50-60 years old group（t=0.954,P〉0.05）.The D-dimer level of 〉60 years old group was significantly different from another two age groups（t=5.972,P〈0.05）.The reference intervals of healthy adults of 18-60 years old and 〉60 years old were 0-225 and 0-297mg/L,respectively.Conclusion The plasma D-dimer level was age-related,which was not associated with gender.The D-dimer level inceased with age.The appropriate biological reference intervals of D-dimer should be established,when the detection objects,instruments and detection reagents were different.