摘要
[Objective] The paper aimed to provide a basis for quality control of compound sulfamonomethoxine sodium propolis solution. [Method] High per- formance liquid chromatography （HPLC） method was used to determine the contents of sulfamonomethoxine sodium and trimethoprim simultaneously in compound sulfamonomethoxine sodium propolis solution. [Result] Sulfamonomethoxine sodium and trimethoprim showed a linear relationship within the concentration of 10 - 500 μg/mL under the following chromatographic conditions： Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, mobile phase 0.02 mol/L phosphoric acid solution- methanol（80：20, V/V）, detection wavelength 270 nm, flow rate 1.0 mL/min, and column temperature 30℃. The average recoveries were 99.3% and 99.4%, respectively. [ Conclusion] The method is accurate and feasible, and can determine sulfamonomethoxine sodium and trimethoprim simultaneously in compound sul- famonomethoxine sodium propolis solution.
[Objective] The paper aimed to provide a basis for quality control of compound sulfamonomethoxine sodium propolis solution. [Method] High per- formance liquid chromatography （HPLC） method was used to determine the contents of sulfamonomethoxine sodium and trimethoprim simultaneously in compound sulfamonomethoxine sodium propolis solution. [Result] Sulfamonomethoxine sodium and trimethoprim showed a linear relationship within the concentration of 10 - 500 μg/mL under the following chromatographic conditions： Kromasil C18 column （250 mm × 4.6 mm, 5 μm）, mobile phase 0.02 mol/L phosphoric acid solution- methanol（80：20, V/V）, detection wavelength 270 nm, flow rate 1.0 mL/min, and column temperature 30℃. The average recoveries were 99.3% and 99.4%, respectively. [ Conclusion] The method is accurate and feasible, and can determine sulfamonomethoxine sodium and trimethoprim simultaneously in compound sul- famonomethoxine sodium propolis solution.
