摘要
目的研究白细胞介素-32(interleukin-32,IL-32)是否增强自然杀伤细胞(naturalkillercells,NKcells)细胞对HepG22.15细胞(插入HBV全基因组,持续表达)的杀伤。方法将IL-32加入HepG22.15细胞培养液中培养48h,然后加入不同浓度的NK细胞与之共培养4h,再加入CCK-8试剂孵育,4h后用酶标仪检测A值,计算NK细胞的杀伤率。接下来将不同浓度的IL-32加入HepG22.15细胞培养液中培养48h.然后加入NK细胞与之共培养4h,再加入CCK.8试剂孵育,检测A值,计算NK细胞的杀伤率。结果IL-32可增强不同浓度的NK细胞对HepG22.15细胞的杀伤,NK细胞与HepG22.15细胞的比例为50:1时杀伤活性最强。IL-32呈剂量依赖性增强NK细胞对HepG22.15细胞的杀伤。结论IL-32可增强NK细胞对HepG22.15细胞的杀伤,可能具有抗HBV的作用及参与HBV感染后的肝损伤。
Objective To investigate whether IL-32 can increase the cytotoxic effect of natural killer (NK) ceils on HepG2 2. 15 ceils. Methods HepG2 2. 15 cells were cultured and treated with IL-32 for 48 h and then with different concentrations of NK cells for 4 h. The ahsorbance (A) was detected and the cytotoxicity rate calculated 4 h after addition of CCK-8. Moreover, different concentrations of IL-32 were used to treat HepG2 2. 15 ceils for 48 h and then NK ceils were co-cul- tured with HepG2 2. 15 cells for 4 h. The A value was detected and the cytotoxicity rate calculat- ed. Results IL-32 increased the cytotoxicity of different concentrations of NK cells to HepG2 2. 15 cells. The cytotoxicity was strongest when the ratio of NK cells to HepG2 2. 15 cells was 50 " 1. IL- 32 enhanced the cytotoxic effect of NK cells on HepG2 2. 15 cells in a dose-dependent man- ner. Conclusion IL-32 can increase the cytotoxic effect of NK cells on HepG2 2. 15 cells, and IL- 32 has anti-HBV effects and participates in the liver injury after HBV infection.
出处
《医学分子生物学杂志》
CAS
2015年第6期311-313,共3页
Journal of Medical Molecular Biology
基金
国家自然科学基金青年基金(No.81401306)