极化液对内毒素血症大鼠肠损伤的影响

Effect of glucose-insulin-potassium on intestinal injury in endotoxemic rats

目的 评价极化液对内毒素血症大鼠肠损伤的影响.方法 清洁级健康雄性SD大鼠60只,体重200～ 250 g,采用随机数字表法分为2组（n=30）：内毒素血症组（LPS组）和极化液组（GIK组）.腹腔注射脂多糖8 mg/kg,1次/d,共注射3次,制备内毒素血症模型.LPS组首次腹腔注射脂多糖2h后静脉输注生理盐水4 ml· kg-1·h-1;GIK组静脉输注极化液4 ml·kg-1·h-1.分别于制模前、制模后3和5d时每组随机取10只大鼠取腹主动脉血样,采用ELISA法测定血浆TNF-α、IL-10和二胺氧化酶（DAO）浓度,并计算TNF-α/IL-10比值,随后处死大鼠取回肠末端,光镜下观察病理学结果并行Chiu评分.结果 与制模前比较,2组制模后3d时血浆TNF-α/IL-10比值、DAO浓度和肠组织Chiu评分升高,LPS组制模后5d时血浆TNF-α/IL-10比值、DAO浓度和肠组织Chiu评分升高,GIK组制模后5d时血浆DAO浓度和肠组织Chiu评分升高（P＜0.05）;与制模后3d时比较,LPS组制模后5d时血浆TNF-α/IL-10比值和DAO浓度升高,GIK组TNF-α/IL-10比值和DAO浓度降低（P＜0.05）;与LPS组比较,GIK组制模后3和5d时血浆TNF-α/IL-10比值、DAO浓度及肠组织Chiu评分降低（P＜0.05）.结论 极化液可减轻内毒素血症致大鼠的肠损伤.

Objective To evaluate the effect of glucose-insulin-potassium （GIK） on intestinal injury in endotoxemic rats.Methods Sixty male Sprague-Dawley rats, weighing 200-250 g, were equally and randonly divided into endotoxemia group （lipopolysaccharide [LPS] group） and GIK group.LPS 8 mg/kg was injected intraperitoneally once a day for 3 times in total to establish the model of endotoxemia-caused intestinal injury.Starting from 2 h after the initial injection of LPS, normal saline was continuously infused at 4 ml · kg 1 · h-t in group LPS, and GIK 4 ml · kg 1 · h 1was infused intravenously in group GIK.Before establishment of the model, and at 3 and 5 days after establishment of the model, 10 rats in each group were sacrificed, and blood samples were collected from the abdominal aorta for determination of tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） and diamine oxidase （DAO） concentrations in plasma by enzyme-linked immunosorbent assay.TNF-α/IL-10 ratio was calculated.A segment of ileum of 2 cm in length, 20 cm from the ileocecal junction, was removed for microscopic examination.The degree of damage to the intestinal mucous membrane was scored according to Chiu.Results Compared with the values before establishment of the model, the plasma TNF-α/IL-10 ratio, DAO concentration, and Chiu＇s scores were significantly increased at 3 days after establishment of the model in the two groups, the plasma TNF-α/IL-10 ratio, DAO concentration, and Chiu＇s scores were increased at 5 days after establishment of the model in group LPS, and the plasma DAO concentration, and Chiu＇s scores were increased at 5 days after establishment of the model in group GIK （P〈0.05）.Compared with the values at 3 days after establishment of the model, the TNF-α/IL-10 ratio and plasma DAO concentration were significantly increased in group LPS, and the TNF-α/IL-10 ratio and plasma DAO concentration were decreased in group GIK at 5 days after establishment of the model in group GIK （P〈0.05）.Compared with group LPS, the TNF-α/IL-10 ratio, plasma DAO concentration, and Chiu＇s scores were significantly decreased at 3 and 5 days after establishment of the model in group G1K （P〈0.05）.Conclusion GIK can reduce intestinal injury in endotoxemic rats.