二溴乙腈对L5178Y细胞的遗传毒性及促凋亡作用

Genotoxicity and apoptosis in L5178Y cells induced by dibromo acetonitrile

目的:研究饮用水中消毒副产物二溴乙腈对小鼠淋巴瘤L5178Y细胞的遗传毒性及促凋亡作用。方法:以L5178Y细胞为靶细胞,采用胞质分裂阻滞微核组学法(CBMN-cyt)和流式细胞术(FCM)分别检测不同浓度(0.1、1、5和10μmol/L)二溴乙腈对L5178Y细胞的遗传毒性及凋亡诱导作用。结果:与阴性对照比较,1和5μmol/L二溴乙腈染毒L5178Y细胞的微核率显著升高(P<0.05);1和10μmol/L二溴乙腈染毒L5178Y细胞的核质桥率显著升高(P<0.05);10μmol/L二溴乙腈染毒L5178Y细胞的核芽率升高(P<0.05);5和10μmol/L二溴乙腈染毒L5178Y细胞的核分裂指数率显著下降(P<0.05)。二溴乙腈各剂量组L5178Y细胞凋亡率均明显高于对照组,差异均具有统计学意义(P均<0.05)。结论:二溴乙腈可致L5178Y细胞遗传毒性并诱导其凋亡。

OBJECTIVE：To study the genotoxicity and apoptosis of the mice L5178 Y lymphoma cells which was induced by disinfection by-product dibromo acetonitrile in drinking water.METHODS：Cytokinesis-block micronucleus cytome assay（CBMN-cyt） and flow cytometry were used to evaluate genotoxicity and apoptosis in mice L5178 Y lymphoma cells treated with different concentrations of dibromo acetonitrile（0.1,1,5 and 10 mol/L）.RESULTS：Compared with the negative control group,frequency of micronucleus（MN） of L5178 Y lymphoma cells in 1 and 5 μmol/L groups increased significantly（P〈0.05）.The nucleoplasmic bridges（NPBs） in the 1 and 10 μmol/L treatment groups and the frequency of nuclear buds（NBUDs） in the 10 μmol/L treatment group all increased significantly（P〈0.05）.However the nuclear divided index（NDI） in the 5 and 10 μmol/L treatment groups all decreased significantly（P〈0.05）.Statistically significant increase of L5178 Y lymphoma cell apoptosis were observed in all dibromo acetonitrile treatment groups in comparison to the negative control group（P〈0.05）.CONCLUSION：Disinfection by-product dibromo acetonitrile in drinking water could obviously induce genotoxicity and apoptosis in L5178 Y lymphoma cells.