摘要
目的:研究饮用水中消毒副产物二溴乙腈对小鼠淋巴瘤L5178Y细胞的遗传毒性及促凋亡作用。方法:以L5178Y细胞为靶细胞,采用胞质分裂阻滞微核组学法(CBMN-cyt)和流式细胞术(FCM)分别检测不同浓度(0.1、1、5和10μmol/L)二溴乙腈对L5178Y细胞的遗传毒性及凋亡诱导作用。结果:与阴性对照比较,1和5μmol/L二溴乙腈染毒L5178Y细胞的微核率显著升高(P<0.05);1和10μmol/L二溴乙腈染毒L5178Y细胞的核质桥率显著升高(P<0.05);10μmol/L二溴乙腈染毒L5178Y细胞的核芽率升高(P<0.05);5和10μmol/L二溴乙腈染毒L5178Y细胞的核分裂指数率显著下降(P<0.05)。二溴乙腈各剂量组L5178Y细胞凋亡率均明显高于对照组,差异均具有统计学意义(P均<0.05)。结论:二溴乙腈可致L5178Y细胞遗传毒性并诱导其凋亡。
OBJECTIVE:To study the genotoxicity and apoptosis of the mice L5178 Y lymphoma cells which was induced by disinfection by-product dibromo acetonitrile in drinking water.METHODS:Cytokinesis-block micronucleus cytome assay(CBMN-cyt) and flow cytometry were used to evaluate genotoxicity and apoptosis in mice L5178 Y lymphoma cells treated with different concentrations of dibromo acetonitrile(0.1,1,5 and 10 mol/L).RESULTS:Compared with the negative control group,frequency of micronucleus(MN) of L5178 Y lymphoma cells in 1 and 5 μmol/L groups increased significantly(P〈0.05).The nucleoplasmic bridges(NPBs) in the 1 and 10 μmol/L treatment groups and the frequency of nuclear buds(NBUDs) in the 10 μmol/L treatment group all increased significantly(P〈0.05).However the nuclear divided index(NDI) in the 5 and 10 μmol/L treatment groups all decreased significantly(P〈0.05).Statistically significant increase of L5178 Y lymphoma cell apoptosis were observed in all dibromo acetonitrile treatment groups in comparison to the negative control group(P〈0.05).CONCLUSION:Disinfection by-product dibromo acetonitrile in drinking water could obviously induce genotoxicity and apoptosis in L5178 Y lymphoma cells.
出处
《癌变.畸变.突变》
CAS
CSCD
2015年第6期450-453,458,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
二溴乙腈
遗传毒性
凋亡
胞质分裂阻滞微核组学
流式细胞术
dibromo acetonitrile
genotoxicity
apoptosis
cytokinesis-block micronucleus cytome assays
flow cytometry