慢性乙型肝炎患者血清白细胞介素33与HBV DNA及ALT水平的相关性分析

Correlation of serum interleukin-33 with HBV DNA load and ALT level in patients with chronic hepatitis B

目的分析慢性乙型肝炎患者的血清白细胞介素(IL)33的水平,探讨其与HBV DNA载量及ALT水平的相关性。方法选取2014年2-11月在上海交通大学医学院附属瑞金医院就诊的慢性乙型肝炎患者119例,选取同期来院的131例健康者作为对照,通过酶联免疫吸附试验测定其血清IL-33水平。对慢性乙型肝炎患者通过实时荧光定量PCR测定其HBV DNA载量(取对数值),通过连续监测法测定其ALT水平。计量资料组间比较采用t检验,血清IL-33水平与HBV DNA载量及ALT水平的相关性采用Pearson相关分析。结果慢性乙型肝炎组患者血清IL-33水平为(62.7±29.3)ng/ml,健康对照组为(16.4±4.5)ng/ml,两组比较差异有统计学意义(t=17.02,P<0.01)。在慢性乙型肝炎患者中,血清IL-33水平与HBV DNA载量呈负相关(r=-0.703,P<0.01),与ALT水平呈正相关(r=0.324,P<0.01)。结论在慢性乙型肝炎患者体内,IL-33作为一种免疫刺激因子,可能对HBV的复制具有抑制作用。

Objective To investigate the serum interleukin - 33 （ IL - 33 ） level in patients with chronic hepatitis B, and to explore the correlation of serum IL - 33 with hepatitis B virus （HBV） DNA load and alanine aminotransferase （ALT） level. Methods A total of 119 patients with chronic hepatitis B and 131 healthy controls were enrolled in this study. Serum levels of IL - 33 were determined by enzyme - linked immunosorbent assay. For patients with chronic hepatitis B, HBV DNA loads were quantified by real - time quantitative PCR, while ALT levels were measured by continuous monitoring assays. The t test was applied in the comparison of continuous data. The Pearson correlation analysis was used to investigate the correlation of serum IL - 33 level with HBV DNA load and ALT level. Results Serum levels of IL - 33 were significantly higher in patients with chronic hepatitis B （ 62.7 ± 29.3 ng/ml） than in healthy controls （ 16.4± 4.5 ng/ml） （ t = 17. 02, P 〈0.01 ）. Serum IL -33 level was negatively correlated with log10 [ HBV DNA] （ r = -0. 703, P 〈0.01 ） but positively correlated with ALT （ r = 0. 324, P 〈 0.01 ） in patients with chronic hepatitis B. Conclusion This study suggests that IL - 33, an immune stimulator, may play a role in inhibiting HBV replication in patients with chronic hepatitis B.